Abstract | BACKGROUND:
Asthma is a heterogeneous disease with differences in onset, severity, and inflammation. Bronchial epithelial cells (BECs) contribute to asthma pathophysiology. OBJECTIVE: METHODS:
RNA sequencing was performed on BECs obtained by bronchoscopy from healthy controls (n = 16), patients with mild asthma (n = 17), patients with moderate asthma (n = 5), and patients with severe asthma (n = 17), as well as on BECs from treated and untreated airways of the latter (also 6 months after bronchial thermoplasty) (n = 23). Lipidome and metabolome analyses were performed on cultured BECs from healthy controls (n = 7); patients with severe asthma (n = 9); and, for comparison, patients with chronic obstructive pulmonary disease (n = 7). RESULTS: Transcriptome analysis of BECs from patients showed a reduced expression of oxidative phosphorylation (OXPHOS) genes, most profoundly in patients with severe asthma but less profoundly and more heterogeneously in patients with mild asthma. Genes related to fatty acid metabolism were significantly upregulated in asthma. Lipidomics revealed enhanced levels of lipid species ( phosphatidylcholines, lysophosphatidylcholines. and bis(monoacylglycerol)phosphate), whereas levels of OXPHOS metabolites were reduced in BECs from patients with severe asthma. BECs from patients with mild asthma characterized by hyperresponsive production of mediators implicated in neutrophilic inflammation had decreased expression of OXPHOS genes compared with that in BECs from patients with mild asthma with normoresponsive production. BECs obtained after thermoplasty had significantly increased expression of OXPHOS genes and decreased expression of fatty acid metabolism genes compared with BECs obtained from untreated airways. CONCLUSION:
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Authors | Abilash Ravi, Annika W M Goorsenberg, Annemiek Dijkhuis, Barbara S Dierdorp, Tamara Dekker, Michel van Weeghel, Yanaika S Sabogal Piñeros, Pallav L Shah, Nick H T Ten Hacken, Jouke T Annema, Peter J Sterk, Frédéric M Vaz, Peter I Bonta, René Lutter |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 148
Issue 5
Pg. 1236-1248
(11 2021)
ISSN: 1097-6825 [Electronic] United States |
PMID | 33556463
(Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved. |
Topics |
- Adolescent
- Adult
- Aged
- Asthma
(metabolism, pathology, therapy)
- Bronchi
(pathology)
- Bronchial Thermoplasty
- Female
- Gene Expression Profiling
- Healthy Volunteers
- Humans
- Lipid Metabolism
(genetics)
- Male
- Middle Aged
- Oxidative Phosphorylation
- Respiratory Mucosa
(metabolism, pathology)
- Severity of Illness Index
- Young Adult
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