Curcumin Efficacy in a Serum/Glucose Deprivation-Induced Neuronal PC12 Injury Model.

Abstract

BACKGROUND:

Glucose/serum deprivation (GSD), has been used for understanding molecular mechanisms of neuronal damage during ischemia. It has been suggested that curcumin may improve neurodegenerative diseases.

AIM:

In this study, the protective effects of curcumin and its underlying mechanisms were investigated in PC12 cells upon GSD-induced stress.

METHODS:

PC12 cells were cultured in DMEM overnight and then incubated in GSD condition for either 6 or 12h. GSD-treated cells were pretreated with various concentrations of curcumin (10, 20, and 40 μM) for 5h. The cell viability, apoptosis, reactive oxygen species (ROS) level, oxidative stress, expression of apoptosis-related genes, and IL-6 were determined.

RESULTS:

Curcumin increased cell viability and caused an anti-apoptotic effect in PC12 cells exposed for 12h to GSD . Curcumin also increased antioxidant enzyme expression, suppressed lipid peroxidation, and decreased interleukin-6 secretion in PC12 cells subjected to GSD. In addition, pretreatment with curcumin down-regulated pro-apoptotic (Bax), and up-regulated antiapoptotic (Bcl2) mediators.

CONCLUSION:

Curcumin mitigates many of the adverse effects of ischemia, and therefore, should be considered as an adjunct therapy in ischemic patients.

• Authors: Tahereh Farkhondeh, Milad Ashrafizadeh, Mohsen Azimi-Nezhad, Fariborz Samini, Michael Aschner, Saeed Samarghandian
• Journal: Current molecular pharmacology (Curr Mol Pharmacol) Vol. 14 Issue 6 Pg. 1146-1155 ( 2021) ISSN: 1874-4702 [Electronic] United Arab Emirates
• PMID: 33538682 (Publication Type: Journal Article)
• Copyright: Copyright© Bentham Science Publishers; For any queries, please email at [email protected].

Chemical References

• Curcumin
• Glucose

Topics

• Animals
• Apoptosis
• Curcumin (pharmacology, therapeutic use)
• Glucose (pharmacology)
• Humans
• Oxidative Stress
• PC12 Cells
• Rats