Protocol for a mouse CNS demyelination model induced by a combination of HSV-1 and IL-2.

Abstract

Multiple sclerosis (MS) is an autoimmune demyelinating disease of the central nervous system. Although viruses have been suggested to be a contributing environmental factor, conventional experimental MS mouse models do not account for this aspect. Here, we describe a mouse model to induce and evaluate demyelinating disease with both a viral and an immune component via ocular infection with a recombinant herpes simplex virus expressing murine interleukin-2. For complete details on the use and execution of this protocol, please refer to Hirose et al. (2020).

• Authors: Satoshi Hirose, Kati Tormanen, Mihoko Kato, Jack Yu, Homayon Ghiasi
• Journal: STAR protocols (STAR Protoc) Vol. 2 Issue 1 Pg. 100287 (03 19 2021) ISSN: 2666-1667 [Electronic] United States
• PMID: 33521681 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: © 2020 The Authors.

Chemical References

• Interleukin-2

Topics

• Animals
• Cell Line
• Disease Models, Animal
• Herpes Simplex (genetics, immunology)
• Herpesvirus 1, Human (immunology)
• Interleukin-2 (adverse effects, immunology, pharmacology)
• Mice
• Mice, Knockout
• Multiple Sclerosis (genetics, immunology)
• Rabbits