Abstract | BACKGROUND: METHODS: We developed a method to study thermal preference of oxaliplatin-treated mice and assessed analgesic activity of intraperitoneal duloxetine and pregabalin used at 30 mg/kg. A prototype analgesiameter and a broad range of temperatures (0-45 °C) were used. Advanced methods of image analysis (deep learning and machine learning) enabled us to determine the effectiveness of analgesics. The loss or reversal of thermal preference of oxaliplatin-treated mice was a measure of analgesia. RESULTS:
Duloxetine selectively attenuated cold-induced pain at temperatures between 0 and 10 °C. Pregabalin-treated mice showed preference towards a colder plate of the two used at temperatures between 0 and 45 °C. CONCLUSION: Unlike duloxetine, pregabalin was not selective for temperatures below thermal preferendum. It influenced pain sensation at a much wider range of temperatures applied. Therefore, for the attenuation of cold hypersensitivity duloxetine seems to be a better than pregabalin therapeutic option. We propose wide-range measurements of thermal preference as a novel method for the assessment of analgesic activity in mice.
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Authors | Kinga Sałat, Anna Furgała-Wojas, Michał Awtoniuk, Robert Sałat |
Journal | Molecules (Basel, Switzerland)
(Molecules)
Vol. 26
Issue 3
(Jan 25 2021)
ISSN: 1420-3049 [Electronic] Switzerland |
PMID | 33503911
(Publication Type: Journal Article)
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Chemical References |
- Analgesics
- Antineoplastic Agents
- Oxaliplatin
- Pregabalin
- Duloxetine Hydrochloride
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Topics |
- Analgesics
(pharmacology)
- Animals
- Antineoplastic Agents
(pharmacology)
- Cold Temperature
- Disease Models, Animal
- Duloxetine Hydrochloride
(pharmacology)
- Hot Temperature
- Hyperalgesia
(drug therapy)
- Male
- Mice
- Oxaliplatin
(pharmacology)
- Pain
(drug therapy)
- Pain Measurement
(methods)
- Pregabalin
(pharmacology)
- Temperature
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