Abstract | BACKGROUND: METHODS: RESULTS: FaDu cells pretreated with MRP1 inhibitors exhibited significantly higher radioactivity than those without inhibitor treatment ( cyclosporine A: 6.91 ± 0.27, lapatinib: 10.03 ± 0.47, MK-571: 10.15 ± 0.44%dose/mg protein, p < 0.01). In the in vivo PET study, the SUVmean ratio in tumors [calculated as after treatment (2nd PET scan)/before treatment of MRP1 inhibitors (1st PET scan)] of the mice treated with MRP1 inhibitors was significantly higher than those of control mice ( cyclosporine A: 2.6 ± 0.7, lapatinib: 2.2 ± 0.7, MK-571: 2.2 ± 0.7, control: 1.2 ± 0.2, p < 0.05). CONCLUSION: In this study, we revealed that MRP1 inhibitors increase [18F] FMISO accumulation in hypoxic cells. This suggests that [18F] FMISO-PET imaging is affected by MRP1 inhibitors independent of the hypoxic state.
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Authors | Yoichi Shimizu, Yukihiro Nakai, Hiroyuki Watanabe, Shimpei Iikuni, Masahiro Ono, Hideo Saji, Yuji Kuge, Tsuneo Saga, Yuji Nakamoto |
Journal | EJNMMI research
(EJNMMI Res)
Vol. 11
Issue 1
Pg. 9
(Jan 25 2021)
ISSN: 2191-219X [Print] Germany |
PMID | 33492449
(Publication Type: Journal Article)
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