RNA methyltransferase METTL3 induces intrinsic resistance to gefitinib by combining with MET to regulate PI3K/AKT pathway in lung adenocarcinoma.

Abstract

Clinical research data show that gefitinib greatly improves the progression-free survival of patients, so it is used in advanced non-small cell lung cancer patients with EGFR mutation. However, some patients with EGFR sensitive mutations do not have good effects on initial gefitinib treatment, and this mechanism is rarely studied. METTL3, a part of N6-adenosine-methyltransferase, has been reported to play an important role in a variety of tumours. In this study, we found that METTL3 is up-regulated in gefitinib-resistant tissues compared to gefitinib-sensitive tissues. Cell function experiments have proved that under the treatment of gefitinib, METTL3 knockdown promotes apoptosis and inhibits proliferation of lung cancer cells. Mechanistic studies have shown that METTL3 combines with MET and causes the PI3K/AKT signalling pathway to be manipulated, which affects the sensitivity of lung cancer cells to gefitinib. Therefore, our research shows that METTL3 can be used as a molecular marker to predict the efficacy of EGFR-TKI therapy in patients, and METTL3 may be a potential therapeutic target.

• Authors: Fangyan Gao, Qianqian Wang, Chang Zhang, Chen Zhang, Tianyu Qu, Jingya Zhang, Jifu Wei, Renhua Guo
• Journal: Journal of cellular and molecular medicine (J Cell Mol Med) Vol. 25 Issue 5 Pg. 2418-2425 (03 2021) ISSN: 1582-4934 [Electronic] England
• PMID: 33491264 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

Chemical References

• Methyltransferases
• METTL3 protein, human
• MET protein, human
• Proto-Oncogene Proteins c-met
• Proto-Oncogene Proteins c-akt
• Gefitinib

Topics

• Adenocarcinoma of Lung
• Cell Line, Tumor
• Drug Resistance, Neoplasm (genetics)
• Gefitinib (pharmacology)
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• Humans
• Methylation
• Methyltransferases (genetics, metabolism)
• Phosphatidylinositol 3-Kinases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• Proto-Oncogene Proteins c-met (metabolism)
• Signal Transduction (drug effects)