Post-treatment with yiqifumai injection and its main ingredients attenuates lipopolysaccharide-induced microvascular disturbance in mesentery and ileum.

Abstract

OBJECTIVE:

To investigate the effect of Yiqifumai injection (YQFM), a compound Chinese medicine, and its main active ingredients on lipopolysaccharide (LPS)-induced microvascular disturbance in mesentery and ileum.

METHODS:

Rats were infused with LPS (5 mg/kg/h) for 90 min. Thirty minutes after initiation of LPS administration, YQFM (160 mg/kg/h), Rb1 (5 mg/kg/h), Sch (2.5 mg/kg/h), or Rb1+Sch (5 mg/kg/h + 2.5 mg/kg/h) was infused until 90 min. Human umbilical vein endothelial cells (HUVECs) were incubated with LPS (100 ng/ml) for 90 min. YQFM (1 mg/ml), Rb1 (100 µM), Sch (100 µM), or Rb1+Sch (200 µM) was added 30 min after initiation of LPS stimulation.

RESULTS:

Yiqifumai injection and Rb1+Sch inhibited mesenteric venule hyperpermeability, suppressed microvillar erosion and submucosal edema, and protected claudin-5 from downregulation and interleukin-1β from upregulation in ileal tissues after LPS. Study in HUVECs confirmed the effect of YQFM and Rb1+Sch on JAM-1 after LPS and revealed a similar effect on other junction proteins. Moreover, YQFM and Rb1+Sch attenuated the dysfunctional energy metabolism and the activation of TLR-4/Src/NF-κB signaling with Rb1 and Sch being partially effective.

CONCLUSION:

These results demonstrated the beneficial effect of post-treatment with YQFM, which is attributable to its main ingredient Rb1 and Sch, and likely mediated by targeting TLR-4/Src/NF-κB signaling pathway.

• Authors: Ayan Ayididaer, Kai Sun, Chun-Shui Pan, Li Yan, Yu-Ying Liu, Dan-Tong Li, Jing-Yu Fan, Jing-Yan Han
• Journal: Microcirculation (New York, N.Y. : 1994) (Microcirculation) Vol. 28 Issue 4 Pg. e12680 (05 2021) ISSN: 1549-8719 [Electronic] United States
• PMID: 33486837 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2021 John Wiley & Sons Ltd.

Chemical References

• Cardiovascular Agents
• Drugs, Chinese Herbal
• Lipopolysaccharides
• NF-kappa B
• Toll-Like Receptor 4
• yi-qi-fu-mai

Topics

• Animals
• Cardiovascular Agents (administration & dosage)
• Drugs, Chinese Herbal (administration & dosage)
• Human Umbilical Vein Endothelial Cells (drug effects)
• Humans
• Ileum (blood supply)
• Lipopolysaccharides (toxicity)
• Mesentery (blood supply)
• Microvessels (drug effects)
• NF-kappa B
• Rats
• Toll-Like Receptor 4
• Vascular Diseases (drug therapy, etiology)