Rising cases of Non
melanoma skin
carcinoma (NMSC) and escalating levels of ultraviolet radiations have underlined a profound correlation with the elevating levels of environmental detoriation and increasing health issues. However, the availability of
therapeutics has not aided in controlling the recurrence rates of skin
carcinoma. Frequent administration of
therapeutics with higher chances of facial
deformity escalates the patient's treatment expenses. Thus, this study initiates a low cost effective and biodegradable
therapy by exploring four formulations with combinations of
silver nanoparticles (AgNPs),
sericin (isolated from cocoons of Antherea mylitta) and
chitosan. Subsequently, various ethosomal formulations were evaluated as a platform for transdermal delivery vehicle for efficient skin intervention
therapeutics. Characterization using UV visible spectroscopy, Dynamic light scattering, Fourier Infrared spectroscopy, X-ray dispersion, Transmission electron microscopy, Fluorescence assisted cell sorting and in vitro studies were done and it was inferenced that equal combination of AgNPs and
sericin facilitated to combat the morphological and cellular deformation of the epidermoid A431skin
carcinoma cells. The overproduction of
superoxide (O2.) and
nitric oxide (NO) radicals consequently depolarized the mitochondrial membrane potential triggering apoptosis and
necrosis. The in vivo experiments exhibited the stimulation of
IgM secretion with T cell-mediated immune response. Therefore, this study proposes a novel approach for treatment of NMSC using biocompatible formulations delivered through ethosomes.