Abstract | BACKGROUND: METHODS: Western blotting, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), Counting Kit-8 assay, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, luciferase activity assay immunofluorescence, and immunohistochemistry were used to analyze the regulatory effects of miR-218 on bone neoplasms cells. RESULTS: Here, the results showed that transfection of miR-128 suppressed bone neoplasms cells proliferation, migration, and invasion. Genetic knockdown of miR-128 in bone neoplasms cells suppressed the activation of the Wnt/β- catenin and epithelial-mesenchymal transition (EMT) signaling pathways. Activation of Wnt or EMT blocked miR-128-inhibited cells proliferation and migration in bone neoplasms cells. Exogenously introduced miR-128 markedly inhibited tumor regeneration in bone neoplasms xenograft models. CONCLUSIONS: These results define a tumor-regulated function for miR-128 in bone neoplasms by down-regulation of the Wnt/β- catenin and EMT signal pathways, which provided a potential target for bone neoplasms gene therapy.
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Authors | Yang Li, Xiaotao Long, Ji Wang, Jing Peng, Kai Shen |
Journal | Journal of orthopaedic surgery and research
(J Orthop Surg Res)
Vol. 16
Issue 1
Pg. 71
(Jan 20 2021)
ISSN: 1749-799X [Electronic] England |
PMID | 33472642
(Publication Type: Journal Article)
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Chemical References |
- MicroRNAs
- Organic Cation Transport Proteins
- Wnt Proteins
- beta Catenin
- solute carrier family 22 (organic cation transporter), member 3
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Topics |
- Animals
- Bone Neoplasms
(genetics, pathology, therapy)
- Cell Line, Tumor
- Cell Movement
(genetics)
- Cell Proliferation
(genetics)
- Disease Models, Animal
- Down-Regulation
(genetics)
- Female
- Humans
- Male
- Mice, Nude
- MicroRNAs
(physiology, therapeutic use)
- Middle Aged
- Molecular Targeted Therapy
- Neoplasm Transplantation
- Organic Cation Transport Proteins
(metabolism)
- Signal Transduction
(genetics, physiology)
- Wnt Proteins
(metabolism)
- beta Catenin
(metabolism)
- Mice
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