Epithelial to Mesenchymal Transition(EMT) Regulated by MicroRNA-429 in Pancreatic Ductal Adenocarcinoma (PDAC).

Abstract

In this study, we aimed to investigate the role of miRNA-429 in the pathogenesis of pancreatic ductal adenocarcinoma(PDAC) and the potential mechanism of this procedure. Totally 95 consecutive patients with PDAC diagnosed by pathology were retrospectively collected from June 1st , 2015 to August 30th, 2019 in Department of General Surgery, Jingmen First People's Hospital. The human pancreatic cancer cell line Bxpc-3 and Panc-1 were used and the cell proliferation and migration were detected by MTT assays and Transwell assays, respectively. The quantitative real-time polymerase chain reaction (qRT-PCR) was applied to evaluate the expression in RNA level. Our results showed that overexpression of miRNA-429 could suppress the cell invasion, proliferation and metastasis through regulating the process of EMT in PDAC cell line, while low expression of miRNA-429 had the opposite effects. We demonstrated that miRNA-429 had critical roles in the pathogenesis of PDAC. Clinically, we observed that tumor tissues from patients with PDAC exhibited significantly decreasing in miRNA-429 expression compared with the non-tumor tissues. Additionally, decreased expression of miRNA-429 in tumor tissues of patients with PDAC was associated with poorer prognosis and several clinical-pathological characteristics. In conclusion, miRNA-429 exerted anti-tumor functions in PDAC through the regulation of EMT process. The results of this study would provide a novel insight into tumorigenesis and the basis for the development of miRNA-targeting therapies against PDAC.

• Authors: Gang Zheng, Zhigang Wang, Jie Tang, Yin Sun, Zhou Hu, Huiping Chen
• Journal: Bioscience reports (Biosci Rep) (Jan 18 2021) ISSN: 1573-4935 [Electronic] England
• PMID: 33458739 (Publication Type: Journal Article)
• Copyright: Copyright 2021 The Author(s).