Abstract |
PD-L1 immune checkpoint inhibitor expression was evaluated in high-grade serous carcinoma (HGSC) ovary in the context of the overall immune landscape to determine its prognostic value. Consecutive cases of HGSC, 50 who underwent upfront surgery followed by adjuvant chemotherapy (HGSC-U) and 50 who underwent neoadjuvant chemotherapy (NACT) followed by interval debulking surgery (HGSC-PC) were selected. In HGSC-PC cases, the pre-NACT ascitic fluid cell blocks were included. Tumor-infiltrating lymphocytes (TILs) were scored, hotspots chosen for tissue microarray construction and immunohistochemistry performed and scored for CD4 and CD8 lymphocyte subsets, CD68+ tumor-associated macrophages (TAMs), PD-1 and PD-L1 expression. HGSC-post- chemotherapy showed increased TILs, predominantly CD8+T-lymphocytes, compared to HGSC-U. HGSC showed PD-L1 expression on tumor cells and/or TAMs in 60% cases with a linear correlation to CD4+, CD8+ TIL levels. Concordant PD-L1 expression was seen in matched pre- and post-NACT tumor cells. HGSC-PC showed higher expression of PD-L1. There was no association of PD-L1 cumulative proportion score or tumor cell score with outcome. Taking a cutoff for PD-L1 CPS at 10%, immunotype I (PD-L1+/CD-8+), corresponding to tumors with adaptive immune evasion, showed worst disease-free survival compared to all other immunotypes (p = 0.03) and was more significant (p = 0.01) when compared to immunotype III (PD-L1+/CD8-). Immunotyping based on PD-L1/CD8+ expression correlates to prognosis and outcome.
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Authors | Akriti Bansal, Radhika Srinivasan, Manish Rohilla, Bhavana Rai, Arvind Rajwanshi, Vanita Suri, Subhas Chandra Saha |
Journal | APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
(APMIS)
Vol. 129
Issue 5
Pg. 254-264
(May 2021)
ISSN: 1600-0463 [Electronic] Denmark |
PMID | 33455015
(Publication Type: Journal Article)
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Copyright | © 2021 APMIS. Published by John Wiley & Sons Ltd. |
Chemical References |
- B7-H1 Antigen
- Biomarkers, Tumor
- CD274 protein, human
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Topics |
- Adult
- Aged
- B7-H1 Antigen
(metabolism)
- Biomarkers, Tumor
(metabolism)
- CD8-Positive T-Lymphocytes
(metabolism, pathology)
- Chemotherapy, Adjuvant
- Cystadenocarcinoma, Serous
(drug therapy, immunology, pathology, surgery)
- Cytoreduction Surgical Procedures
- Disease-Free Survival
- Female
- Humans
- Immunophenotyping
- Lymphocytes, Tumor-Infiltrating
(metabolism, pathology)
- Middle Aged
- Neoadjuvant Therapy
- Ovarian Neoplasms
(drug therapy, immunology, pathology, surgery)
- Prognosis
- T-Lymphocyte Subsets
(metabolism, pathology)
- Tumor Microenvironment
(immunology)
- Tumor-Associated Macrophages
(metabolism, pathology)
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