Delphinidin Increases the Sensitivity of Ovarian Cancer Cell Lines to 3-Bromopyruvate.

Abstract

3-Bromopyruvic acid (3-BP) is a promising anticancer compound. Two ovary cancer (OC) cell lines, PEO1 and SKOV3, showed relatively high sensitivity to 3-BP (half maximal inhibitory concentration (IC50) of 18.7 and 40.5 µM, respectively). However, the further sensitization of OC cells to 3-BP would be desirable. Delphinidin (D) has been reported to be cytotoxic for cancer cell lines. We found that D was the most toxic for PEO1 and SKOV3 cells from among several flavonoids tested. The combined action of 3-BP and D was mostly synergistic in PEO1 cells and mostly weakly antagonistic in SKOV3 cells. The viability of MRC-5 fibroblasts was not affected by both compounds at concentrations of up to 100 µM. The combined action of 3-BP and D decreased the level of ATP and of dihydroethidium (DHE)-detectable reactive oxygen species (ROS), cellular mobility and cell staining with phalloidin and Mitotracker Red in both cell lines but increased the 2',7'-dichlorofluorescein (DCFDA)-detectable ROS level and decreased the mitochondrial membrane potential and mitochondrial mass only in PEO1 cells. The glutathione level was increased by 3-BP+D only in SKOV3 cells. These differences may contribute to the lower sensitivity of SKOV3 cells to 3-BP+D. Our results point to the possibility of sensitization of at least some OC cells to 3-BP by D.

• Authors: Natalia Pieńkowska, Grzegorz Bartosz, Paulina Furdak, Izabela Sadowska-Bartosz
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 22 Issue 2 (Jan 12 2021) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 33445795 (Publication Type: Journal Article)

Chemical References

• Anthocyanins
• Antineoplastic Agents
• Flavonoids
• Pyruvates
• Reactive Oxygen Species
• dihydroethidium
• bromopyruvate
• Adenosine Triphosphate
• delphinidin
• Ethidium
• Glutathione

Topics

• Adenosine Triphosphate (metabolism)
• Anthocyanins (pharmacology)
• Antineoplastic Agents (pharmacology)
• Cell Line
• Cell Line, Tumor
• Cell Movement (drug effects)
• Cell Survival (drug effects)
• Drug Synergism
• Ethidium (analogs & derivatives, pharmacology)
• Female
• Fibroblasts (drug effects, metabolism)
• Flavonoids (pharmacology)
• Glutathione (metabolism)
• Humans
• Membrane Potential, Mitochondrial (drug effects)
• Mitochondria (drug effects, metabolism)
• Ovarian Neoplasms (drug therapy, metabolism)
• Pyruvates (pharmacology)
• Reactive Oxygen Species (metabolism)