Lung cancer is the most common
cancer type worldwide and the leading cause of
cancer-related mortality. Diabetes is closely associated with the occurrence, development and prognosis of
lung cancer. Therefore, the present study aimed to investigate whether SNCG could affect the proliferation of
lung cancer cells induced by high
glucose.
Lung cancer cells induced by high
glucose simulated the pathologies of patients with
lung cancer with diabetes in vitro. The proliferation of HBE cells and
lung cancer cells after transfection and treatment of
glucose was detected using Cell Counting Kit-8 assay. The
mRNA expression levels of
synuclein γ (SNCG),
insulin-like growth factor 1 (IGF-1) and
IGF-1 receptor (IGF-1R) in HBE cells and
lung cancer cells alone, or cells induced by high
glucose were analyzed via reverse transcription-quantitative (RT-q)PCR analysis. Moreover RT-qPCR analysis was used to determine the transfection efficiencies. The clone formation ability, migration and
inflammation of
lung cancer cells after high
glucose induction and transfection were detected using clone formation, wound healing and ELISA assays. The
protein expression levels of SNCG,
IGF-1, IGF-1R, ERK 1/2, phosphorylated (p)-ERK1/2 and JNK in
lung cancer cells after high
glucose induction and transfection were determined using western blot analysis. The results suggested that high
glucose significantly promoted the proliferation of A549, NCI-H1975 and SK-MES-1 cells at 24 and 48 h, as well as upregulated the expression levels of SNCG,
IGF-1 and IGF-1R. Knockdown of SNCG suppressed the proliferation, clone formation ability and migration, but alleviated
inflammation in A549 cells induced by high
glucose. Knockdown of SNCG suppressed the expression levels of SNCG,
IGF-1, IGF-1R, ERK1/2 and p-ERK1/2, while it promoted JNK expression in A549 cells induced by high
glucose. The effect of
AXL1717 (an IGF-1R inhibitor) treatment on cells was consistent with that of SNCG knockdown. In conclusion, inhibition of SNCG suppresses proliferation of
lung cancer cells induced by high
glucose.