Abstract |
Activation of PRR ([ pro]renin receptor) contributes to enhancement of intrarenal RAS and renal medullary α-ENaC and thus elevated blood pressure during Ang II ( angiotensin II) infusion. The goal of the present study was to test whether such action of PRR was mediated by sPRR (soluble PRR), generated by S1P (site-1 protease), a newly identified PRR cleavage protease. F1 B6129SF1/J mice were infused for 6 days with control or Ang II at 300 ng/kg per day alone or in combination with S1P inhibitor PF-429242 (PF), and blood pressure was monitored by radiotelemetry. S1P inhibition significantly attenuated Ang II-induced hypertension accompanied with suppressed urinary and renal medullary renin levels and expression of renal medullary but not renal cortical α-ENaC expression. The effects of S1P inhibition were all reversed by supplement with histidine-tagged sPRR termed as sPRR-His. Ussing chamber technique was performed to determine amiloride-sensitive short-circuit current, an index of ENaC activity in confluent mouse cortical collecting duct cell line cells exposed for 24 hours to Ang II, Ang II + PF, or Ang II + PF + sPRR-His. Ang II-induced ENaC activity was blocked by PF, which was reversed by sPRR-His. Together, these results support that S1P-derived sPRR mediates Ang II-induced hypertension through enhancement of intrarenal renin level and activation of ENaC.
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Authors | Ye Feng, Kexin Peng, Renfei Luo, Fei Wang, Tianxin Yang |
Journal | Hypertension (Dallas, Tex. : 1979)
(Hypertension)
Vol. 77
Issue 2
Pg. 405-416
(02 2021)
ISSN: 1524-4563 [Electronic] United States |
PMID | 33280408
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- PF-429242
- Pyrrolidines
- Receptors, Cell Surface
- Angiotensin II
- Proprotein Convertases
- Serine Endopeptidases
- membrane-bound transcription factor peptidase, site 1
- Renin
- Prorenin Receptor
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Topics |
- Angiotensin II
(pharmacology)
- Animals
- Blood Pressure
(drug effects)
- Hypertension
(chemically induced, genetics, metabolism)
- Mice
- Proprotein Convertases
(antagonists & inhibitors)
- Pyrrolidines
(pharmacology)
- Receptors, Cell Surface
(genetics, metabolism)
- Renin
(metabolism)
- Renin-Angiotensin System
(drug effects)
- Serine Endopeptidases
- Prorenin Receptor
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