Curcumin-loaded niosomes downregulate mRNA expression of pro-inflammatory markers involved in asthma: an in vitro study.

Abstract

Aim: In this study, curcumin was encapsulated in niosomes (Nio-Curc) to increase its effectiveness for the treatment of asthma. Materials & methods: The formulation underwent various physicochemical characterization experiments, an in vitro release study, molecular simulations and was evaluated for in vitro anti-inflammatory activity. Results: Results showed that Nio-Curc had a mean particle size of 284.93 ± 14.27 nm, zeta potential of -46.93 and encapsulation efficacy of 99.62%, which demonstrates optimized physicochemical characteristics. Curcumin release in vitro could be sustained for up to 24 h. Additionally, Nio-Curc effectively reduced mRNA transcript expression of pro-inflammatory markers; IL-6, IL-8, IL-1β and TNF-α in immortalized human airway basal cell line (BCi-NS1.1). Conclusion: In this study, we have demonstrated that Nio-Curc mitigated the mRNA expression of pro-inflammatory markers in an in vitro study, which could be applied to treatment of asthma with further studies.

• Authors: Jin-Ying Wong, Zhao Yin Ng, Meenu Mehta, Shakti D Shukla, Jithendra Panneerselvam, Thiagarajan Madheswaran, Gaurav Gupta, Poonam Negi, Pradeep Kumar, Viness Pillay, Alan Hsu, Nicole G Hansbro, Peter Wark, Mary Bebawy, Philip Michael Hansbro, Kamal Dua, Dinesh Kumar Chellappan
• Journal: Nanomedicine (London, England) (Nanomedicine (Lond)) Vol. 15 Issue 30 Pg. 2955-2970 (12 2020) ISSN: 1748-6963 [Electronic] England
• PMID: 33252322 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Liposomes
• RNA, Messenger
• Curcumin

Topics

• Asthma (drug therapy)
• Curcumin (pharmacology)
• Humans
• Liposomes
• Particle Size
• RNA, Messenger (genetics)