Abstract | BACKGROUND:
Diabetic foot ulcer is the most costly and complex challenge for patients with diabetes. We hereby assessed the effectiveness of different preconditioned adipose-derived mesenchymal stem cells (AD-MSCs) and photobiomodulation protocols on treating an infected ischemic wound in type 1 diabetic rats. METHODS: There were five groups of rats: (1) control, (2) control AD-MSCs [diabetic AD-MSCs were transplanted (grafted) into the wound bed], (3) AD-MSC + photobiomodulation in vivo (diabetic AD-MSCs were grafted into the wound, followed by in vivo PBM treatment), (4) AD-MSCs + photobiomodulation in vitro, and (5) AD-MSCs + photobiomodulation in vitro + in vivo. RESULTS: Diabetic AD-MSCs preconditioned with photobiomodulation had significantly risen cell function compared to diabetic AD-MSC. Groups 3 and 5 had significantly decreased microbial flora correlated to groups 1 and 2 (all, p = 0.000). Groups 2, 3, 4, and 5 had significantly improved wound closure rate (0.4, 0.4, 0.4, and 0.8, respectively) compared to group 1 (0.2). Groups 2-5 had significantly increased wound strength compared to group 1 (all p = 0.000). In most cases, group 5 had significantly better results than groups 2, 3, and 4. CONCLUSIONS: Preconditioning diabetic AD-MSCs with photobiomodulation in vitro plus photobiomodulation in vivo significantly hastened healing in the diabetic rat model of an ischemic infected delayed healing wound.
|
Authors | Houssein Ahmadi, Abdollah Amini, Fatemeh Fadaei Fathabady, Atarodsadat Mostafavinia, Fatemeh Zare, Roohollah Ebrahimpour-Malekshah, Mustafa Neshat Ghalibaf, Matin Abrisham, Fatemehalsadat Rezaei, Richard Albright, Seyed Kamran Ghoreishi, Sufan Chien, Mohammad Bayat |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 11
Issue 1
Pg. 494
(11 25 2020)
ISSN: 1757-6512 [Electronic] England |
PMID | 33239072
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
|
Topics |
- Animals
- Diabetes Mellitus, Experimental
(therapy)
- Humans
- Low-Level Light Therapy
- Mesenchymal Stem Cell Transplantation
- Rats
- Rats, Wistar
- Stem Cells
- Wound Healing
|