The MicroRNA-210/Casp8ap2 Pathway Alleviates Hypoxia-Induced Injury in Myocardial Cells by Regulating Apoptosis and Autophagy.

Abstract

AIM:

This study was conducted to investigate the role of the miR-210/Caspase8ap2 pathway in apoptosis and autophagy in hypoxic myocardial cells.

METHODS:

The miR-control, miR-210 mimic, and miR-210 inhibitor were transfected into rat myocardial H9C2 cells. The transfection efficiency of exogenous miR-210 was determined by quantitative reverse-transcription polymerase chain reaction (qRT-PCR). H9C2 cells were then treated with CoCl2 for 24, 48, and 72 h to generate a myocardial injury model. The apoptosis of H9C2 cells was assessed by flow cytometry. Additionally, a western blot assay was used to determine the expression of the autophagy-associated proteins light chain 3 (LC3), p62 and Beclin-1, and apoptosis-associated proteins Caspase8ap2, cleaved caspase 8, and cleaved caspase 3.

RESULTS:

We determined that a 48 h hypoxia treatment duration in H9C2 cardiomyocytes induced myocardial injury. Additionally, the overexpression of miR-210 significantly inhibited cell apoptosis. MiR-210 suppressed autophagy by upregulating p62 and downregulating LC3II/I in hypoxic H9C2 cells. Caspase8ap2 was a putative target of miR-210, miR-210 mediated apoptosis, and autophagy of H9C2 cells via suppressing Caspase8ap2. Furthermore, the expression of caspase 8, caspase 3, and Beclin-1 were decreased in response to miR-210.

CONCLUSION:

miR-210 exhibits anti-apoptosis and anti-autophagy effects, which alleviate myocardial injury in response to hypoxia.

• Authors: Kunsheng Li, Jun Pan, Qiuchang Li, Shiliang Li, Kai Li, Yongqing Cheng, Lin Chai, Chao Li, Junling Li, Zhikun Fu, Dongjin Wang, Yang Bai
• Journal: The heart surgery forum (Heart Surg Forum) Vol. 23 Issue 6 Pg. E797-E802 (Oct 21 2020) ISSN: 1522-6662 [Electronic] United States
• PMID: 33234216 (Publication Type: Journal Article)

Chemical References

• Apoptosis Regulatory Proteins
• CASP8AP2 protein, rat
• MIRN210 microRNA, rat
• MicroRNAs

Topics

• Animals
• Apoptosis
• Apoptosis Regulatory Proteins (biosynthesis, genetics)
• Autophagy
• Blotting, Western
• Cell Line
• Disease Models, Animal
• Gene Expression Regulation
• MicroRNAs (biosynthesis, genetics)
• Myocardial Reperfusion Injury (genetics, metabolism, pathology)
• Myocytes, Cardiac (metabolism, pathology)
• Rats
• Signal Transduction