Abstract | BACKGROUND: METHODS: HUVEC were pre-incubated with colchicine 10 μM for 1 h and then stimulated with oxLDL (50 μg/mL). TF gene (RT-PCR), protein (western blot), surface expression (FACS) and procoagulant activity (FXa generation assay) were measured. TF translocation to cell surface was investigated by immunofluorescence. NF-κB/IκB axis was examined by western blot analysis and translocation assay. Finally, LOX-1 expression was also investigated. RESULTS:
Colchicine significantly reduced TF gene and protein expression as well as its procoagulant activity in oxLDL-treated HUVEC. These effects seem to be related mainly to action of colchicine on microtubules that, in turn, modulate TF trafficking in the cytoplasm, NF-κB/IκB pathway and LOX-1 expression. CONCLUSIONS: Data of the present study, although in vitro, indicate that one of the hypothetical mechanisms by which colchicine exert protective cardiovascular effects might be its ability to inhibit the pro-thrombotic activity of oxLDL.
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Authors | Giovanni Cimmino, Stefano Conte, Andrea Morello, Grazia Pellegrino, Laura Marra, Gaetano Calì, Paolo Golino, Plinio Cirillo |
Journal | Vascular pharmacology
(Vascul Pharmacol)
Vol. 137
Pg. 106822
(04 2021)
ISSN: 1879-3649 [Electronic] United States |
PMID | 33232770
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Elsevier Inc. All rights reserved. |
Chemical References |
- Fibrinolytic Agents
- I-kappa B Proteins
- Lipoproteins, LDL
- NF-kappa B
- OLR1 protein, human
- Scavenger Receptors, Class E
- oxidized low density lipoprotein
- Thromboplastin
- Factor Xa
- Colchicine
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Topics |
- Blood Coagulation
(drug effects)
- Cells, Cultured
- Colchicine
(pharmacology)
- Factor Xa
(metabolism)
- Fibrinolytic Agents
(pharmacology)
- Human Umbilical Vein Endothelial Cells
(drug effects, metabolism)
- Humans
- I-kappa B Proteins
(metabolism)
- Lipoproteins, LDL
(pharmacology)
- NF-kappa B
(metabolism)
- Scavenger Receptors, Class E
(genetics, metabolism)
- Thromboplastin
(genetics, metabolism)
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