Hsa_circ_0001869 promotes NSCLC progression via sponging miR-638 and enhancing FOSL2 expression.

Abstract

Accumulating studies suggest that circular RNAs (circRNAs) function as key regulators in human cancers. We found that hsa_circ_0001869 participated in non-small cell lung cancer (NSCLC) progression. However, its expression and function during NSCLC remain unknown. The data advised that hsa_circ_0001869 expression was increased in NSCLC cell lines and tissues. High hsa_circ_0001869 expression had negatively correlation with the NSCLC patients prognosis. Bioinformatics and luciferase report analyses confirmed that miR-638 and FOSL2 were hsa_circ_0001869 downstream target. hsa_circ_0001869 downregulation decreased tumor proliferation, invasion and migration by promoting miR-638 expression and decreasing FOSL2 expression. As a result of overexpression of FOSL2 or silencing of miR-638, the recovery of proliferation, migration, and invasion after hsa_circ_0001869 silencing. Overexpression of FOSL2 also led to recovery of migration, invasion and proliferation after upregulation of miR-638. In vivo studies confirmed that overexpression of FOSL2 or silencing of miR-638 led to the recovery of tumor growth ability regarding A549 cells after hsa_circ_0001869 knockdown. Present investigation discovered that hsa_circ_0001869 enhanced NSCLC progression via sponging miR-638 and promoting FOSL2 expression. hsa_circ_0001869 downregulation suppressed tumor growth and invasion ability.

• Authors: Pei Xu, Lei Wang, Xiao Xie, Fengqing Hu, Qi Yang, Rui Hu, Lianyong Jiang, Fangbao Ding, Ju Mei, Jianhui Liu, Haibo Xiao
• Journal: Aging (Aging (Albany NY)) Vol. 12 Issue 23 Pg. 23836-23848 (11 20 2020) ISSN: 1945-4589 [Electronic] United States
• PMID: 33221767 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• FOSL2 protein, human
• Fos-Related Antigen-2
• MIRN638 microRNA, human
• MicroRNAs
• RNA, Circular

Topics

• A549 Cells
• Animals
• Carcinoma, Non-Small-Cell Lung (genetics, metabolism, pathology)
• Cell Movement
• Cell Proliferation
• Computational Biology
• Databases, Genetic
• Disease Progression
• Female
• Fos-Related Antigen-2 (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (genetics, metabolism, pathology)
• Male
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Neoplasm Invasiveness
• RNA, Circular (genetics, metabolism)
• Signal Transduction
• Tumor Burden