Lactobacillus plantarum BSGP201683 Improves the Intestinal Barrier of Giant Panda Microbiota-Associated Mouse Infected by Enterotoxigenic Escherichia coli K88.

Abstract

Giant pandas often suffered from gastrointestinal disease, especially the captive sub-adult one. Our study aims to investigate whether L. plantarum G83, a good panda-derived probiotic, can improve the intestinal barrier against the enterotoxigenic Escherichia coli K88 (E. coli K88) infection in giant panda microbiota-associated mice (GPAM). We treated SPF mice with antibiotics cocktail and transplanted the giant panda intestinal microbiota to set up a GPAM. Our results demonstrated that the microbiota of GPAM changed over time and was relatively stable in the short-term experiment (2-4 weeks). Whereafter, the GPAM pretreated with L. plantarum G83 for 15 days and infected with enterotoxigenic E. coli K88. The result indicated that the number of Bifidobacteria spp. increased in GPAM-G and GPAM-GE groups; the Lactobacillus spp. only increased in the GPAM-G group. Although the abundance of Enterobacteriaceae spp. only decreased in the GPAM-G group, the copy number of Escherichia coli in the GPAM-E group was significantly lower than that in the other groups. Meanwhile, the L. plantarum G83-induced alteration of microbiota could increase the mRNA expression of Claudin-1, Zo-1, and Occludin-1 in the GPAM-G group in the ileum; only Occludin-1 was increased in the GPAM-GE group. The sIgA in the ileum showed a positive response, also the result of body weight and histology in both the GPAM-G and GPAM-GE group. These results indicated that the L. plantarum G83 could improve the intestinal barrier to defense the enterotoxigenic E. coli K88 invasion.

• Authors: Yi Zhou, Xueqin Ni, Ling Duan, Lili Niu, Qian Liu, Yan Zeng, Qiang Wang, Jie Wang, Abdul Khalique, Kangcheng Pan, Bo Jing, Dong Zeng
• Journal: Probiotics and antimicrobial proteins (Probiotics Antimicrob Proteins) Vol. 13 Issue 3 Pg. 664-676 (06 2021) ISSN: 1867-1314 [Electronic] United States
• PMID: 33190214 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Occludin

Topics

• Animals
• Enterotoxigenic Escherichia coli
• Escherichia coli Infections (veterinary)
• Gastrointestinal Microbiome
• Intestines (microbiology)
• Lactobacillus plantarum
• Mice
• Occludin
• Ursidae (microbiology)