Detection and characterisation of sheep-associated malignant catarrhal fever infection from ruminants by using tegument and gB gene sequences of OvHV-2.

Abstract

In this study, positive blood and organ samples were obtained from different mixed herds of sheep and cattle against ovine herpesvirus 2 (OvHV-2) infection. Target-positive DNA was sequenced and compared with worldwide distributed OvHV-2 sequences. Tegument gene (422 base pairs) and glycoprotein B (gB) gene (2800 base pairs) amplicons of OvHV-2 genome were used for understanding of epidemiology of malignant catarrhal fever (MCF) infection in Turkey. The results of nucleotide sequencing of polymerase chain reaction (PCR) products indicated presence of sheep-associated form for MCF infection in Turkey. Although the obtained sequences were genetically different from each other, it was found that genetic variations were limited.

• Authors: Tuba Ç Oğuzoğlu, Seçkin Salar, Ece Adıgüzel, Cansu Demirden, Onur Ülgenalp
• Journal: The Onderstepoort journal of veterinary research (Onderstepoort J Vet Res) Vol. 87 Issue 1 Pg. e1-e4 (Nov 11 2020) ISSN: 2219-0635 [Electronic] South Africa
• PMID: 33179949 (Publication Type: Journal Article)

Chemical References

• Viral Proteins

Topics

• Animals
• Cattle
• Female
• Gammaherpesvirinae (genetics, isolation & purification)
• Malignant Catarrh (diagnosis, virology)
• Sequence Analysis, DNA (veterinary)
• Sheep
• Sheep Diseases (diagnosis, virology)
• Sheep, Domestic
• Turkey
• Viral Proteins (genetics)