Abstract | OBJECTIVE: METHODS: A traditional cell model of AD was established by inducing N2a cells with Aβ25-35, and the optimal Aβ25-35 concentration was determined by assessing the cell viability changes. Another cell model of AD was established by transfecting N2a cells with SORL1-shRNA lentiviral vector, and SORL1 expression in the transfected cells were detected using Western blotting and qRT-PCR. With wild-type N2a cells without any treatment and cells transfected with a scramble shRNA as the control groups, the two cell models were examined for cell viability with MTT assay, cell apoptosis with flow cytometry, and TNF-α and IL -1β levels in the culture supernatant with ELISA. RESULTS: The two cell models of AD showed obviously decreased viability and increased cell apoptosis compared with the untreated control cells or cells transfected with a scramble shRNA (P < 0.05); no significant difference was found in the cell viability and apoptosis rate between the two AD cell models or between the two control groups (P>0.05). Significantly increased expressions of TNF-α and IL-1β were observed in both of the two cell models compared with their respective control groups (P < 0.05) without significant differences between the two cell models or between the two control groups (P>0.05). CONCLUSIONS: A new AD cell model similar to Aβ25-35-induced AD model can be established by SORL1 knockdown in N2a cells.
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Authors | Jing Luo, Yan Zhao, Jingwen Xie, Xin Liu, Fangbo Lin, Deren Hou |
Journal | Nan fang yi ke da xue xue bao = Journal of Southern Medical University
(Nan Fang Yi Ke Da Xue Xue Bao)
Vol. 38
Issue 1
Pg. 8-13
(Jan 30 2018)
ISSN: 1673-4254 [Print] China |
PMID | 33177021
(Publication Type: English Abstract, Journal Article)
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