[Silencing of SLP-2 inhibits the migration and invasion of cervical cancer cells in vitro].

Abstract

OBJECTIVE:

To investigate the effect of SLP-2 silencing on the migration and invasion of human cervical cancer cells and explore the mechanism.

METHODS:

Small interfering RNA (siRNA) was used to knockdown the expression of SLP-2 in Hela cells and Siha cells. At 48 h after the transfection, the cells were examined for SLP-2 expression with Western blotting, and wound healing assay and Transwell assay were used to evaluate the changes in the cell migration; Matrigel Transwell assay was used to evaluate the changes in the invasion ability of the cells. The expressions of E-cadherin, β-catenin, vimentin and Twist in Hela and Siha cells following the transfection were detected with Western blotting.

RESULTS:

Compared with the control cells, siRNA transfection significantly lowered the expression of SLP-2 and suppressed the migration and invasion ability of Hela cells and Siha cells (P < 0.01). Silencing SLP-2 induced obvious up-regulation of epithelial cell phenotype proteins E-cadherin and β-catenin, down- regulated the expression of interstitial cell phenotype protein vimentin, and lowered the expression of Twist in the cells.

CONCLUSIONS:

Silencing SLP-2 via siRNA transfection can inhibit epithelial-mesenchymal transition of human cervical cancer cells and lower their migration and invasion abilities possibly in relation with downregulated expression of Twist.

• Authors: Jialu Zhang, Guolin Hu, Lei Liu, Huodi Chen, Danjuan Li, Weijiang Liang
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 38 Issue 7 Pg. 812-817 (Jul 30 2018) ISSN: 1673-4254 [Print] China
• PMID: 33168500 (Publication Type: English Abstract, Journal Article)