Abstract | OBJECTIVE: METHODS: Unilateral 6-hydroxydopamine (6-OHDA) lesioned rats received DBS in the STN using an implantable microstimulation system, sham DBS in the STN, or no electrode placement. Continuous unilateral STN-DBS (current intensity 50 µA, frequency 130 Hz, and pulse width 52 µs) was conducted for 14 days. Rats were then sacrificed and brains shock frozen. Striata and motor cortices were dissected with a cryostat. Levels of VEGF, BDNF, and GDNF were analysed, both by quantitative PCR and colorimetric ELISA. RESULTS: PCR revealed a significant upregulation of only BDNF mRNA in the ipsilateral striata of the DBS group, when compared to the sham-stimulated group. There was no significant increase in VEGF mRNA or GDNF mRNA. ELISA analysis showed augmentations of BDNF, VEGF, as well as GDNF protein in the ipsilateral striata after DBS compared to sham stimulation. In the motor cortex, significant increases after DBS were observed for BDNF only, not for the other 2 NFs. CONCLUSIONS: The upregulation of trophic factors induced by STN-DBS may participate in its long-term therapeutic efficacy and potentially neuroprotective effects.
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Authors | Katharina Faust, Peter Vajkoczy, Bai Xi, Daniel Harnack |
Journal | Stereotactic and functional neurosurgery
(Stereotact Funct Neurosurg)
Vol. 99
Issue 3
Pg. 256-266
( 2021)
ISSN: 1423-0372 [Electronic] Switzerland |
PMID | 33152730
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2020 S. Karger AG, Basel. |
Chemical References |
- Brain-Derived Neurotrophic Factor
- Glial Cell Line-Derived Neurotrophic Factor
- Vascular Endothelial Growth Factor A
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Topics |
- Animals
- Brain-Derived Neurotrophic Factor
(genetics, metabolism)
- Deep Brain Stimulation
- Glial Cell Line-Derived Neurotrophic Factor
- Parkinson Disease
(therapy)
- Rats
- Rats, Sprague-Dawley
- Subthalamic Nucleus
(metabolism)
- Vascular Endothelial Growth Factor A
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