Simultaneous determination of six antibiotics in human serum by high-performance liquid chromatography with UV detection.

Abstract

Antibiotics are widely used in intensive care patients to treat severe infections. To avoid bacterial resistance or toxic side effects, the determination of serum concentration of ABs is advisable. Therefore, in this study, we developed and validated a simple and fast high-performance liquid chromatography method with UV detection for the simultaneous determination of four β-lactam ABs (meropenem, imipenem, ceftazidime, and piperacillin) and two coadministered substances (cilastatin and tazobactam) in human serum. Sample preparation required a simple protein precipitation by methanol. The separation of the ABs occurred within a timeframe of 17 min. For this purpose, we used a Kinetex F5 column with a linear gradient of acetonitrile and phosphate buffer (pH 6.9). The UV detector recorded two separate chromatograms at 220 and 295 nm simultaneously. Validation has demonstrated that the method is linear, accurate, and precise within the clinically relevant range for each substance.

• Authors: Thomas Roth, Lea Weber, Marion Niestroj, Franziska Cipa, Astrid Löscher, Sidonia Mihai, Hans Parsch
• Journal: Biomedical chromatography : BMC (Biomed Chromatogr) Vol. 35 Issue 3 Pg. e5010 (Mar 2021) ISSN: 1099-0801 [Electronic] England
• PMID: 33119907 (Publication Type: Journal Article)
• Copyright: © 2020 The Authors. Biomedical Chromatography published by John Wiley & Sons Ltd.

Chemical References

• Anti-Bacterial Agents
• beta-Lactams

Topics

• Anti-Bacterial Agents (blood, chemistry, isolation & purification)
• Chromatography, High Pressure Liquid (methods)
• Drug Monitoring
• Humans
• Limit of Detection
• Linear Models
• Reproducibility of Results
• Spectrophotometry, Ultraviolet
• beta-Lactams (blood, chemistry, isolation & purification)