CD90 Is Dispensable for White and Beige/Brown Adipocyte Differentiation.

Abstract

Brown adipose tissue (BAT) is a thermogenic organ in rodents and humans. In mice, the transplantation of BAT has been successfully used to combat obesity and its comorbidities. While such beneficial properties of BAT are now evident, the developmental and cellular origins of brown, beige, and white adipocytes have remained only poorly understood, especially in humans. We recently discovered that CD90 is highly expressed in stromal cells isolated from human white adipose tissue (WAT) compared to BAT. Here, we studied whether CD90 interferes with brown or white adipogenesis or white adipocyte beiging. We applied flow cytometric sorting of human adipose tissue stromal cells (ASCs), a CRISPR/Cas9 knockout strategy in the human Simpson-Golabi-Behmel syndrome (SGBS) adipocyte model system, as well as a siRNA approach in human approaches supports the hypothesis that CD90 affects brown or white adipogenesis or white adipocyte beiging in humans. Taken together, our findings call the conclusions drawn from previous studies, which claimed a central role of CD90 in adipocyte differentiation, into question.

• Authors: Meike Dahlhaus, Julian Roos, Daniel Engel, Daniel Tews, Daniel Halbgebauer, Jan-Bernd Funcke, Sophie Kiener, Patrick J Schuler, Johannes Döscher, Thomas K Hoffmann, Julia Zinngrebe, Markus Rojewski, Hubert Schrezenmeier, Klaus-Michael Debatin, Martin Wabitsch, Pamela Fischer-Posovszky
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 21 Issue 21 (Oct 24 2020) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 33114405 (Publication Type: Journal Article)

Chemical References

• Thy-1 Antigens

Topics

• Adipose Tissue, Beige (cytology, metabolism)
• Adipose Tissue, Brown (cytology, metabolism)
• Adipose Tissue, White (cytology, metabolism)
• Adult
• Arrhythmias, Cardiac (genetics, metabolism)
• CRISPR-Cas Systems
• Cell Differentiation
• Cells, Cultured
• Female
• Flow Cytometry
• Gene Knockout Techniques
• Genetic Diseases, X-Linked (genetics, metabolism)
• Gigantism (genetics, metabolism)
• Heart Defects, Congenital (genetics, metabolism)
• Humans
• Intellectual Disability (genetics, metabolism)
• Male
• Middle Aged
• Stromal Cells (metabolism)
• Thermogenesis
• Thy-1 Antigens (genetics, metabolism)
• Up-Regulation