MicroRNA-mRNA expression profiles and functional network of submandibular gland in type 2 diabetic db/db mice.

Abstract

OBJECTIVE:

Hyposalivation is a common symptom of diabetes. Although microRNAs (miRNAs) play a role in the pathogenesis of diabetes, the specific effects of miRNAs on diabetic salivary glands are not clear.

DESIGN:

We used high-throughput technologies to screen differentially expressed (DE) miRNAs and mRNAs in submandibular gland (SMG) tissues from db/db mice and db/m mice. DE miRNAs and mRNAs were confirmed using quantitative real-time polymerase chain reaction (qRT-PCR).

RESULTS:

Twenty-eight DE miRNAs and 1146 DE mRNAs were identified between the SMG tissues of db/db mice and db/m mice. Gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated that the DE miRNAs were highly associated with terms related to diverse biological processes and signalling pathways. Of the related pathways, the tight junction pathway, autophagy pathway and transforming growth factor-β (TGF-β) signalling pathway were notable. AKT serine/threonine kinase 3 (AKT3) and phosphoinositide-3 kinase catalytic subunit delta (PIK3CD) may also play important roles in the development of diabetes-mediated hyposalivation.

CONCLUSIONS:

Our research described the miRNA-mRNA expression profiles and miRNA-mRNA network in the SMG tissues of db/db mice. These results provide possible molecular mechanisms of diabetes-induced hyposalivation and information for further studies.

• Authors: Hui-Min Liu, Yan Huang, Li Li, Yan Zhang, Xin Cong, Li-Ling Wu, Ruo-Lan Xiang
• Journal: Archives of oral biology (Arch Oral Biol) Vol. 120 Pg. 104947 (Dec 2020) ISSN: 1879-1506 [Electronic] England
• PMID: 33113460 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• MicroRNAs
• RNA, Messenger

Topics

• Animals
• Diabetes Mellitus, Experimental (genetics)
• Diabetes Mellitus, Type 2 (genetics)
• Gene Expression Profiling
• Gene Regulatory Networks
• Mice
• MicroRNAs (genetics)
• RNA, Messenger (genetics)
• Submandibular Gland