Structural Insights into the Interactions of Candidal Enolase with Human Vitronectin, Fibronectin and Plasminogen.

Abstract

Significant amounts of enolase-a cytosolic enzyme involved in the glycolysis pathway-are exposed on the cell surface of Candida yeast. It has been hypothesized that this exposed enolase form contributes to infection-related phenomena such as fungal adhesion to human tissues, and the activation of fibrinolysis and extracellular matrix degradation. The aim of the present study was to characterize, in structural terms, the protein-protein interactions underlying these moonlighting functions of enolase. The tight binding of human vitronectin, fibronectin and plasminogen by purified C. albicans and C. tropicalis enolases was quantitatively analyzed by surface plasmon resonance measurements, and the dissociation constants of the formed complexes were determined to be in the 10-7-10-8 M range. In contrast, the binding of human proteins by the S.cerevisiae enzyme was much weaker. The chemical cross-linking method was used to map the sites on enolase molecules that come into direct contact with human proteins. An internal motif 235DKAGYKGKVGIAMDVASSEFYKDGK259 in C. albicans enolase was suggested to contribute to the binding of all three human proteins tested. Models for these interactions were developed and revealed the sites on the enolase molecule that bind human proteins, extensively overlap for these ligands, and are well-separated from the catalytic activity center.

• Authors: Dorota Satala, Grzegorz Satala, Justyna Karkowska-Kuleta, Michal Bukowski, Anna Kluza, Maria Rapala-Kozik, Andrzej Kozik
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 21 Issue 21 (Oct 22 2020) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 33105833 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Fibronectins
• Immobilized Proteins
• Vitronectin
• Plasminogen
• Phosphopyruvate Hydratase

Topics

• Amino Acid Motifs
• Antibodies (metabolism)
• Binding, Competitive
• Candida albicans (enzymology)
• Candida tropicalis (enzymology)
• Cytosol (enzymology)
• Fibronectins (chemistry, metabolism)
• Host-Pathogen Interactions (physiology)
• Humans
• Immobilized Proteins (metabolism)
• Models, Molecular
• Phosphopyruvate Hydratase (chemistry, genetics, immunology, metabolism)
• Plasminogen (chemistry, metabolism)
• Vitronectin (chemistry, metabolism)