Volume
resuscitation is an important early treatment for haemorrhagic
shock. Haemoglobin-based
oxygen carrier (HBOC) can expand the volume and provide
oxygen for tissues. Vascular leakage is common complication in the process of haemorrhagic
shock and
resuscitation. The aim of this study was to observe the effects of HBOC (a bovine-derived, cross-linked tetramer haemoglobin
oxygen-carrying
solution, 0.5 g/L) on vascular leakage in rats after haemorrhagic
shock. A haemorrhagic
shock rat model and hypoxic vascular endothelial cells (VECs) were used. The role of intercellular junctions and endothelial glycocalyx in the protective effects of HBOC and the relationship with mitochondrial function were analysed. After haemorrhagic
shock, the pulmonary vascular permeability to
FITC-BSA,
Evans Blue was increased, endothelial glycocalyx was destroyed and the expression of intercellular junction
proteins was decreased. After haemorrhagic
shock, a small volume of HBOC
solution (6 ml/kg) protected pulmonary vascular permeability, increased structural thickness of endothelial glycocalyx, the levels of its components and increased expression levels of the intercellular junction
proteins ZO-1,
VE-cadherin and
occludin. Moreover, HBOC significantly increased
oxygen delivery and consumption in rats, improved VEC mitochondrial function and structure. In conclusion, HBOC mitigates endothelial leakage by protecting endothelial glycocalyx and intercellular junctions through improving mitochondrial function and tissue
oxygen delivery.