The present study compared the effects of ultrasonic irradiation and
SonoVue microbubbles (US) or
Lipofectamine 3000 on the transfection of
small interfering RNA for PRR11 (siPRR11) and
Proline-rich
protein 11 (PRR11) overexpression plasmid into
breast cancer cells. SiPRR11 and PRR11 overexpression plasmid were transfected into
breast cancer MCF7 cells mediated by US and
Lipofectamine 3000. PRR11 expressions in
breast cancer and normal tissues were determined using Gene Expression Profiling Interactive Analysis (GEPIA). The viability, proliferation, migration, invasion and apoptosis of
breast cancer cells were respectively measured by MTT assay, clone formation assay, scratch wound-healing assay, Transwell assay and flow cytometry. PRR11 and epithelial-to-mesenchymal transition (EMT)-related and apoptosis-related (
B-cell lymphoma 2, Bcl-2; Bcl-2-associated
protein X, Bax)
proteins' expressions were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as appropriate. As ultrasonic intensity increased, the viability of MCF7 cells was decreased. Results from GEPIA suggested that PRR11 was up-regulated in
breast cancer. Silencing PRR11 mediated by US showed a higher efficiency than by
Lipofectamine 3000. SiPRR11 transfected by
Lipofectamine 3000 suppressed cells growth and
metastasis, while promoted cell apoptosis. Moreover,
E-cadherin (E-cad) and Bax expressions were high but
N-cadherin (N-cad), Snail and Bcl-2 expressions were low. However, overexpressed PRR11 caused the opposite effects. More importantly, transfection of siPRR11 and PRR11 overexpression plasmid using US had a higher efficacy than using
Lipofectamine 3000. US transfection of PRR11
siRNA showed better effects on inhibiting
breast cancer progression. The current findings contribute to a novel treatment for
breast cancer.