Anti-cancer Evaluation of Depsides Isolated from Indonesian Folious Lichens: Physcia millegrana, Parmelia dilatata and Parmelia aurulenta.

Abstract

Cancer is a serious health burden on global societies. The discovery and development of new anti-cancer therapies remains a challenging objective. Although it has been shown that lichen secondary metabolites may be potent sources for new anti-cancer agents, the Indonesian- grown folious lichens, Physcia millegrana,Parmelia dilatata and Parmeila aurulenta, have not yet been explored. In this study exhaustive preparative high-performance liquid chromatography was employed to isolate the lichen constituents with spectroscopic and spectrometric protocols identifying nine depsides 9-17, including the new methyl 4-formyl-2,3-dihydroxy-6-methylbenzoate 13. The cytotoxicity of the depsides towards cancer cells was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results indicated lowest toxicity of the depsides towards human A549 lung cancer cells. Importantly, the di-depsides (11, 12 and 17) showed greatest toxicity, indicating that these structures are biologically more active than the mono-depsides against the HepG2 liver cancer, A549 lung cancer and HL-60 leukemia cell lines.

• Authors: Ari Satia Nugraha, Tinton Agung Laksono, Lilla Nur Firli, Chintya Permata Zahky Sukrisno Putri, Dwi Koko Pratoko, Zulfikar Zulfikar, Ludmilla Fitri Untari, Hendris Wongso, Jacob M Lambert, Carolyn T Dillon, Paul A Keller
• Journal: Biomolecules (Biomolecules) Vol. 10 Issue 10 (10 08 2020) ISSN: 2218-273X [Electronic] Switzerland
• PMID: 33049949 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Dipeptides
• Fungal Proteins

Topics

• A549 Cells
• Antineoplastic Agents (chemistry, isolation & purification, pharmacology)
• Ascomycota (chemistry)
• Dipeptides (chemistry, isolation & purification, pharmacology)
• Drug Screening Assays, Antitumor
• Fungal Proteins (chemistry, isolation & purification, pharmacology)
• HL-60 Cells
• Hep G2 Cells
• Humans
• Indonesia
• Neoplasms (drug therapy, metabolism, pathology)
• Parmeliaceae (chemistry)