Alteration in angiotensin-converting enzyme 2 by PM1 during the development of emphysema in rats.

Abstract

INTRODUCTION:

Angiotensin-converting enzyme 2 (ACE2) provides an adhesion site for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Patients with COPD could have severe outcomes after SARS-CoV-2 infection. The objective of this study was to investigate ACE2 regulation by air pollution during the development of COPD.

METHODS:

Sprague Dawley rats were exposed to unconcentrated traffic-related air pollution for 3 and 6 months. We examined lung injury markers, oxidative stress, inflammation, emphysema, ACE2 and angiotensin II receptor type 1 (AT1) and 2 (AT2) in the lungs after exposure.

RESULTS:

Lung injury occurred due to an increase in permeability and lactate dehydrogenase cytotoxicity was observed after 6 months of exposure to fine particulate matter of <1 μm in aerodynamic diameter (PM1). An α1-antitrypsin deficiency and neutrophil elastase production with emphysema development were observed after 6 months of PM1 exposure. 8-isoprostane and interleukin-6 were increased after 3 and 6 months of PM1 exposure. Caspase-3 was increased after exposure to PM1 for 6 months. Upregulation of ACE2 was found after 3 months of PM1 exposure; however, ACE2 had decreased by 6 months of PM1 exposure. AT1 and AT2 had significantly decreased after exposure to PM1 for 6 months. Furthermore, smooth muscle hypertrophy had occurred after 6 months of PM1 exposure.

CONCLUSIONS:

In conclusion, short-term exposure to PM1 increased the ACE2 overexpression in lungs. Long-term exposure to PM1 decreased the ACE2 overexpression in emphysema. Air pollution may be a risk for SARS-CoV-2 adhesion during the development of COPD.

• Authors: Hsiao-Chi Chuang, Yi-Ying Chen, Ta-Chih Hsiao, Hsiu-Chu Chou, Han-Pin Kuo, Po-Hao Feng, Shu-Chuan Ho, Jen-Kun Chen, Kai-Jen Chuang, Kang-Yun Lee
• Journal: ERJ open research (ERJ Open Res) Vol. 6 Issue 4 (Oct 2020) ISSN: 2312-0541 [Print] England
• PMID: 33043050 (Publication Type: Journal Article)
• Copyright: Copyright ©ERS 2020.