DLX6-AS1 accelerates cell proliferation through regulating miR-497-5p/SNCG pathway in prostate cancer.

Abstract

Prostate cancer (PCa) has become the second leading cause of cancer-related mortality in males worldwide. Although the long noncoding RNA DLX6-AS1 has been recognized to be an oncogene in multiple cancers, the biological function and regulatory mechanism of DLX6-AS1 in prostate cancer are still obscure. In the present study, we observed that DLX6-AS1 was significantly upregulated in PCa tissues and cells. Knockdown of DLX6-AS1 inhibited PCa progression by suppressing cell proliferation and accelerating cell apoptosis. Molecular mechanism exploration indicated that DLX6-AS1 acted as a sponge for miR-497-5p and synuclein gamma (SNCG) was a downstream target gene of miR-497-5p. In addition, there was a negative correlation between DLX6-AS1 and miR-497-5p in PCa tissues. Rescue assays showed that SNCG overexpression could partially recover DLX6-AS1 knockdown-mediated inhibition of progression in PCa. Furthermore, xenograft tumor model was established to determine the role of DLX6-AS1 in PCa tumor growth and the results suggested that DLX6-AS1 could facilitate tumor growth by regulating SNCG in vivo. In conclusion, our study investigated the biological function and underlying mechanism of DLX6-AS1 in PCa and validated that DLX6-AS1 functioned as an oncogene through miR-497-5p/SNCG axis.

• Authors: Xu Zhu, Xingxin Ma, Shuli Zhao, Zhigang Cao
• Journal: Environmental toxicology (Environ Toxicol) Vol. 36 Issue 3 Pg. 308-319 (Mar 2021) ISSN: 1522-7278 [Electronic] United States
• PMID: 33035382 (Publication Type: Journal Article)
• Copyright: © 2020 Wiley Periodicals LLC.

Chemical References

• DLX6 protein, human
• Homeodomain Proteins
• MIRN497 microRNA, human
• MicroRNAs
• Neoplasm Proteins
• RNA, Long Noncoding
• SNCG protein, human
• gamma-Synuclein

Topics

• Apoptosis
• Cell Proliferation (genetics)
• Disease Progression
• Gene Expression Regulation, Neoplastic
• Homeodomain Proteins (genetics, metabolism)
• Humans
• Male
• MicroRNAs (metabolism)
• Neoplasm Proteins (genetics)
• Prostatic Neoplasms (genetics)
• RNA, Long Noncoding (genetics)
• Up-Regulation
• gamma-Synuclein (genetics, metabolism)