Abstract |
<b>Aim:</b> The purpose of this study was to investigate the oxidative DNA damage, pro- antioxidant status in Polish patients with inflammatory bowel disease (IBD). <br><b>Methods:</b> Oxidative DNA damage was measured by comet assay techniques; nitric oxide (NO) and plasmatic lipid peroxidation (MDA) as oxidative stress were valuated by colometric methods; superoxide dismutase (SOD1), catalase (CAT) and glutathione peroxidase (GPx1) as antioxidative defense were determined by spectrophotometric methods. <br><b>Results:</b> The level of oxidative DNA damage in IBD patients was significantly higher in relation to controls (P = 0.01). Alike, in control subject as well as in patients with IBD, lymphocytes are characterized by complete repair of DNA damage. A significant decrease of SOD (P = 0.031), CAT (P = 0.006), GPx1 (P = 0.001) activity was seen in IBD patients vs control. MDA (P = 0.001) and NO (P = 0.001) concentrations were significantly increased in IBD patients as compared to healthy subjects. <br><b>Conclusions:</b> Our results may be due to the induction of DNA repair genes which may occur at the stage of the pathological changes (IBD) that may be caused by excessive oxidative stress. However, the cause of this relationship, and whether it is direct or indirect, remains to be explored.
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Authors | Małgorzata Mrowicka, Jerzy Mrowicki, Michał Mik, Łukasz Dziki, Adam Dziki, Ireneusz Majsterek |
Journal | Polski przeglad chirurgiczny
(Pol Przegl Chir)
Vol. 92
Issue 5
Pg. 8-15
(May 26 2020)
ISSN: 2299-2847 [Electronic] Poland |
PMID | 33028732
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Biomarkers
- Nitric Oxide
- Glutathione Peroxidase
- Superoxide Dismutase
- Glutathione Transferase
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Topics |
- Adult
- Antioxidants
(metabolism)
- Biomarkers
(blood)
- Case-Control Studies
- DNA Damage
(physiology)
- Female
- Glutathione Peroxidase
(metabolism)
- Glutathione Transferase
(metabolism)
- Humans
- Inflammatory Bowel Diseases
(blood)
- Male
- Middle Aged
- Nitric Oxide
(blood)
- Oxidative Stress
(physiology)
- Poland
- Superoxide Dismutase
(blood)
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