Abstract |
Ectonucleotidases are a broad family of ectoenzymes that play a crucial role in purinergic cell signaling. Ecto- nucleotide pyrophosphatases/ phosphodiesterases ( NPPs) belong to this group and are important drug targets. In particular, NPP1 and NPP3 are known to be druggable targets for treatment of impaired calcification disorders (including pathological aortic calcification) and cancer, respectively. In this study, we investigated a series of sulfonate and sulfamate derivatives of benzofuran and benzothiophene as potent and selective inhibitors of NPP1 and NPP3. Compounds 1c, 1g, 1n, and 1s are the most active NPP1 inhibitors (IC50 values in the range 0.12-0.95 µM). Moreover, compounds 1e, 1f, 1j, and 1l are the most potent inhibitors of NPP3 (IC50 ranges from 0.12 to 0.95 µM). Compound 1d, 1f and 1t are highly selective inhibitors of NPP1 over NPP3, whereas compounds 1m and 1s are found to be highly selective towards NPP3 over NPP1. Structure-activity relationship (SAR) study has been discussed in detailed. With the aid of molecular docking studies, a common binding mode of these compounds and suramin (the standard inhibitor) was revealed, where the sulfonate group acts as a cation-binding moiety that comes in close contact with the zinc ion of the active site. Moreover, cytotoxic evaluation against MCF-7 and HT-29 cancer cell lines revealed that compound 1r is the most cytotoxic towards MCF-7 cell line with IC50 value of 0.19 µM. Compound 1r is more potent and selective against cancer cells than normal cells (WI-38) as compared to doxorubicin.
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Authors | Hanan S Anbar, Randa El-Gamal, Saif Ullah, Seyed-Omar Zaraei, Mariya Al-Rashida, Sumera Zaib, Julie Pelletier, Jean Sévigny, Jamshed Iqbal, Mohammed I El-Gamal |
Journal | Bioorganic chemistry
(Bioorg Chem)
Vol. 104
Pg. 104305
(11 2020)
ISSN: 1090-2120 [Electronic] United States |
PMID | 33017718
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2020 Elsevier Inc. All rights reserved. |
Chemical References |
- Antineoplastic Agents
- Benzofurans
- Enzyme Inhibitors
- Sulfonic Acids
- Thiophenes
- benzothiophene
- sulfamic acid
- Phosphoric Diester Hydrolases
- Pyrophosphatases
- nucleotide pyrophosphatase
- benzofuran
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Topics |
- Antineoplastic Agents
(chemical synthesis, chemistry, pharmacology)
- Benzofurans
(chemical synthesis, chemistry, pharmacology)
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Dose-Response Relationship, Drug
- Drug Screening Assays, Antitumor
- Enzyme Inhibitors
(chemical synthesis, chemistry, pharmacology)
- Humans
- Molecular Docking Simulation
- Molecular Structure
- Phosphoric Diester Hydrolases
(metabolism)
- Pyrophosphatases
(antagonists & inhibitors, metabolism)
- Structure-Activity Relationship
- Sulfonic Acids
(chemical synthesis, chemistry, pharmacology)
- Thiophenes
(chemical synthesis, chemistry, pharmacology)
- Tumor Cells, Cultured
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