Innovative optofluidics and microscopy-based rapid analysis of pathogens.

Abstract

The timely knowledge and prescription of the most suitable antibiotic to treat bacterial infections is critical for the recovery of patients battling life-threatening bacterial infections. Unfortunately, current standard-of-care approaches relies on the empiric prescription of an antibiotic, as determination of the most effective antibiotic requires multiple time-consuming steps. These steps often include culturing of the bacterium responsible for infection and subsequent antibiotic susceptibility testing. Here we introduce an optofluidic technology that allows us to capture bacterial cells efficiently and rapidly from different biological samples and use the captured cells for rapid antibiotic selection thereby bypassing the need to culture the bacterium.

• Authors: Hao Wang, Priyanka Shiveshwarkar, Robert Brzozowski, Arseny Zhdanov, Shulin Shi, Prahathees Eswara, Anna Pyayt
• Journal: Biomedical optics express (Biomed Opt Express) Vol. 11 Issue 9 Pg. 5060-5069 (Sep 01 2020) ISSN: 2156-7085 [Print] United States
• PMID: 33014600 (Publication Type: Journal Article)
• Copyright: © 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.