Exploration of the effect of salvianolate on myocardial infarction in rats based on tandem mass tags.

Abstract

Salvianolate is a compound from traditional Chinese medicine widely used in the treatment of various cardiovascular diseases. This study explored the effects of salvianolate on myocardial infarction and used tandem mass tags (TMT) to discover differentially expressed proteins. Male Sprague Dawley rats were randomly divided into the sham operation group, model group, and salvianolate group. The myocardial infarction model was established by ligating the left anterior descending coronary artery while the sham group had a sham operation. The rats were intraperitoneally injected with 2 ml of 5% glucose once a day, with 48.438 mg/kg/d salvianolate for the rats in the salvianolate group. After 4 weeks, the rats' hemodynamics were measured to evaluate cardiac function, and Masson staining assessed the area of myocardial infarction. TMT analysis was performed and validated by western blot. Salvianolate improved cardiac function after myocardial infarction, reduced the myocardial infarction area, and protected the myocardial tissue. 100 differentially expressed proteins were identified between the sham operation and model groups, salvianolate reversed the expression of 25 of those proteins, that were mainly involved in the metabolism of extracellular collagen matrix and the response to growth factor stimulation. Type I collagen, type V collagen, chymase, β-myosin heavy chain, and A-Raf differential expression were consistent in western blotting. In conclusion, salvianolate had a protective effect on myocardial tissues of rats with myocardial infarction. Several proteins including type I collagen, type V collagen, chymase, β-myosin, and A-Raf may be salvianolate targets for treatment of myocardial infarction.

• Authors: Cheng Chen, Pengli Zhu, Huizhen Yu, Bin Huang, Mengxuan Gui, Xiang Lin, Yibo Bai
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 889 Pg. 173610 (Dec 15 2020) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 33007290 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier B.V. All rights reserved.

Chemical References

• Plant Extracts
• salvianolate

Topics

• Animals
• Computational Biology (methods)
• Hemodynamics (drug effects, physiology)
• Male
• Myocardial Infarction (drug therapy, metabolism)
• Plant Extracts (metabolism, pharmacology, therapeutic use)
• Proteomics (methods)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Tandem Mass Spectrometry (methods)
• Treatment Outcome