Placenta expression of vitamin D and related genes in pregnant women with gestational diabetes mellitus.

Abstract

OBJECTIVES AND METHODS:

A total of 41 GDM and 40 normal glucose tolerance subjects were recruited. Through detecting the level of Serum vitamin D with electrochemical luminescence and vitamin D receptor (VDR) with Enzyme-linked immunosorbent assay (ELISA) in maternal and cord blood, the expression leves of CYP24A1, CYP27B1, VDR protein and mRNA in placenta and umbilical cord with western blotting and RT-PCR, and the DNA methylation levels of CYP24A1 and CYP27B1 gene in placenta with methylation-specific PCR (MSP) and direct bisulfite sequencing (BSP) analysis to explore the potential role of the vitamin D and its related genes in gestational diabetes mellitus (GDM).

RESULTS:

Serum vitamin D concentrations were significantly higher in normal pregnant than women with GDM in maternal blood (P < 0.01) and cord blood (P = 0.014). Compared to the control group, the expression levels of CYP24A1 protein (P < 0.01) and mRNA (P = 0.021) and VDR protein (P = 0.026) and mRNA (P = 0.023) in the GDM group were significantly higher in placenta and umbilical cord tissues (P = 0.015, P < 0.01, P = 0.028, P < 0.01, respectively), while that of CYP27B1 protein (P < 0.01) and mRNA (P = 0.042) was significantly lower (P = 0.022, P = 0.032, respectively). Moreover, partial DNA methylation of CYP24A1 and CYP27B1 genes was observed in both GDM and control groups.

CONCLUSIONS:

Vitamin D deficiency participates in the pathogenesis of GDM, and changes in the expression of genes related to the vitamin D metabolic pathway are closely related to vitamin D levels in the pregnancy and fetus. However, DNA methylation of CYP24A1 and CYP27B1 might not be involved in the pathogenesis of GDM.

• Authors: Yanyan Wang, Tian Wang, Yan Huo, Li Liu, Suxin Liu, Xiaoqian Yin, Runfang Wang, Xingshuang Gao
• Journal: The Journal of steroid biochemistry and molecular biology (J Steroid Biochem Mol Biol) Vol. 204 Pg. 105754 (11 2020) ISSN: 1879-1220 [Electronic] England
• PMID: 32946925 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Receptors, Calcitriol
• VDR protein, human
• Vitamin D
• CYP24A1 protein, human
• Vitamin D3 24-Hydroxylase
• 25-Hydroxyvitamin D3 1-alpha-Hydroxylase
• CYP27B1 protein, human

Topics

• 25-Hydroxyvitamin D3 1-alpha-Hydroxylase (genetics, metabolism)
• Adult
• DNA Methylation
• Diabetes, Gestational (genetics, metabolism)
• Female
• Fetal Blood (metabolism)
• Gene Expression
• Humans
• Placenta (metabolism)
• Pregnancy
• Receptors, Calcitriol (genetics, metabolism)
• Vitamin D (blood, metabolism)
• Vitamin D3 24-Hydroxylase (genetics, metabolism)