Serum anti-LRPAP1 is a common biomarker for digestive organ cancers and atherosclerotic diseases.

Abstract

Some cancers are related to atherosclerotic diseases; therefore, these two types of disease may share some antibody biomarkers in common. To investigate this, a first screening of sera was performed from patients with esophageal squamous cell carcinoma (ESCC) or acute ischemic stroke (AIS) for serological identification of antigens using recombinant cDNA expression cloning (SEREX). The amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) method, which incorporates glutathione donor beads and anti-human IgG acceptor beads, was used to evaluate serum antibody levels. SEREX screening identified low-density lipoprotein receptor-related protein-associated protein 1 (LRPAP1) as a target antigen of serum IgG antibodies in the sera of patients with ESCC or AIS. Antigens, including recombinant glutathione S-transferase-fused LRPAP1 protein, were prepared to examine serum antibody levels. AlphaLISA revealed significantly higher antibody levels against the LRPAP1 protein in patients with solid cancers such as ESCC and colorectal carcinoma and some atherosclerosis-related diseases such as AIS and diabetes mellitus compared with healthy donors. Correlation analysis revealed that the elevated serum antibody levels against LRPAP1 were associated with smoking, a well-known risk factor for both cancer and atherosclerosis. Serum LRPAP1 antibody is therefore a common marker for the early diagnosis of some cancers and atherosclerotic diseases and may reflect diseases caused by habitual smoking.

• Authors: Makoto Sumazaki, Hideaki Shimada, Masaaki Ito, Fumiaki Shiratori, Eiichi Kobayashi, Yoichi Yoshida, Akihiko Adachi, Tomoo Matsutani, Yasuo Iwadate, Seiichiro Mine, Toshio Machida, Ikuo Kamitsukasa, Masahiro Mori, Kazuo Sugimoto, Akiyuki Uzawa, Satoshi Kuwabara, Yoshio Kobayashi, Mikiko Ohno, Eiichiro Nishi, Yoshiro Maezawa, Minoru Takemoto, Koutaro Yokote, Hirotaka Takizawa, Koichi Kashiwado, Hideo Shin, Takashi Kishimoto, Kazuyuki Matsushita, Sohei Kobayashi, Rika Nakamura, Natsuko Shinmen, Hideyuki Kuroda, Xiao-Meng Zhang, Hao Wang, Ken-Ichiro Goto, Takaki Hiwasa
• Journal: Cancer science (Cancer Sci) Vol. 111 Issue 12 Pg. 4453-4464 (Dec 2020) ISSN: 1349-7006 [Electronic] England
• PMID: 32939876 (Publication Type: Journal Article)
• Copyright: © 2020 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

Chemical References

• Biomarkers
• DNA, Complementary
• Immunoglobulin G
• LDL-Receptor Related Protein-Associated Protein
• Neoplasm Proteins

Topics

• Acute Disease
• Biomarkers (blood)
• Colorectal Neoplasms (blood, immunology)
• DNA, Complementary
• Esophageal Neoplasms (blood, immunology)
• Esophageal Squamous Cell Carcinoma (blood, immunology)
• Humans
• Immunoenzyme Techniques
• Immunoglobulin G (blood)
• Ischemic Stroke (blood, immunology)
• LDL-Receptor Related Protein-Associated Protein (immunology)
• Neoplasm Proteins (immunology)