Abstract | BACKGROUND: METHODS: In the current study, db/m and db/db mice were injected with human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) via tail vein injection, intraperitoneal injection, and skeletal muscle injection. Body weight, fasting blood glucose, and the survival rates were monitored. Furthermore, the anti- insulin resistance effects and potential mechanisms of transplanted HUC-MSCs were investigated in db/db mice in vivo. RESULTS: The results showed that HUC-MSC transplantation by skeletal muscle injection was safer compared with tail vein injection and intraperitoneal injection, and the survival rate reached 100% in the skeletal muscle injection transplanted mice. HUC-MSCs can stabilize localization and differentiation in skeletal muscle tissue and significantly ameliorate insulin resistance. Potential regulatory mechanisms are associated with downregulation of inflammation, regulating the balance between PI3K/Akt and ERK/MAPK signaling pathway via PTEN, but was not associated with the IGF-1/IGF-1R signaling pathway. CONCLUSIONS:
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Authors | Guang Chen, Xiao-Yan Fan, Xiao-Peng Zheng, Yue-Lei Jin, Ying Liu, Shuang-Chun Liu |
Journal | Stem cell research & therapy
(Stem Cell Res Ther)
Vol. 11
Issue 1
Pg. 401
(09 16 2020)
ISSN: 1757-6512 [Electronic] England |
PMID | 32938466
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Proto-Oncogene Proteins c-akt
- PTEN Phosphohydrolase
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Topics |
- Animals
- Diabetes Mellitus, Type 2
- Humans
- Insulin Resistance
- Mesenchymal Stem Cell Transplantation
- Mesenchymal Stem Cells
- Mice
- Muscle, Skeletal
(physiology)
- PTEN Phosphohydrolase
(genetics)
- Phosphatidylinositol 3-Kinases
(genetics)
- Proto-Oncogene Proteins c-akt
(genetics)
- Signal Transduction
- Umbilical Cord
(cytology)
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