[Detection of del(17p13) among newly diagnosed multiple myeloma cases using cytoplasmic light chain immunofluorescence combined with FISH and its clinical significance].

Abstract

OBJECTIVE:

To detect chromosomal aberrations by using cytoplasmic light chain immunofluorescence with fluorescence in situ hybridization (cIg-FISH), and to explore the correlation of del(17p13) with clinical characteristics, drug response and prognosis among patients with newly diagnosed multiple myeloma (NDMM).

METHODS:

Clinical data of 198 cases of NDMM was collected. cIg-FISH and a specific probe (TP53) were used to detect karyotypic abnormalities in bone marrow samples derived from the patients. Correlation between karyotypic abnormalities and clinical data was analyzed.

RESULTS:

Nineteen of the 198 patients (9.6%) were found to have a karyotype involving del(17p13). The overall survival (OS) and progression-free survival (PFS) for patients with or without del(17p13) was significantly different (P<0.01). No significant difference was found in OS and PFS between patients carrying a del(17p13) on bortezomib and non-bortezomib regimen (OS: P = 0.873; PFS: P = 0.610).

CONCLUSION:

cIg-FISH is a simple and convenient method for the detection of karyotypic anomalies in multiple myeloma. Del(17p13) is an indicator for poor prognosis for multiple myeloma patients. Bortezomib cannot improve the survival disadvantage of del(17p13).

• Authors: Xiupan Lu, Lijuan Chen, Qinglin Shi, Hairong Qiu, Jianyong Li, Rui Guo
• Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (Zhonghua Yi Xue Yi Chuan Xue Za Zhi) Vol. 37 Issue 10 Pg. 1087-1091 (Oct 10 2020) ISSN: 1003-9406 [Print] China
• PMID: 32924107 (Publication Type: Journal Article)

Topics

• Chromosome Deletion
• Chromosomes, Human, Pair 17
• Fluorescent Antibody Technique
• Humans
• In Situ Hybridization, Fluorescence
• Karyotyping
• Multiple Myeloma (diagnosis, genetics)
• Prognosis