Decreased serum netrin-1 is associated with ischemic stroke: A case-control study.

Abstract

BACKGROUND AND AIMS:

Netrin-1 was a laminin-related protein involved in neurovascular protection, and we previously discovered that decreased serum netrin-1 was associated with poor prognosis of ischemic stroke. However, the relationship between serum netrin-1 level and the risk of ischemic stroke remains unclear. The aim of this study was to investigate the association between netrin-1 level and risk of ischemic stroke.

METHODS AND RESULTS:

A case-control study including 591 ischemic stroke patients and 591 age- and sex-matched healthy individuals was conducted, and serum netrin-1 concentrations were quantitatively determined via enzyme-linked immunosorbent assay for all participants. The serum netrin-1 levels were significantly lower in the ischemic stroke patients than those in matched controls (median, 496.4 vs 652.1 pg/mL; P < 0.001). After adjustment for potential confounders, the odds ratio of ischemic stroke associated with the highest quartile of netrin-1 was 0.07 (95% CI: 0.01-0.65; Ptrend = 0.018) compared with the lowest quartile of netrin-1. Each 1-standard deviation increase of log-transformed netrin-1 was associated with a lower odds of ischemic stroke (odds ratio: 0.45, 95% CI: 0.22-0.94; P = 0.032), and a dose-response relationship between serum netrin-1 and ischemic stroke was observed (Plinearity = 0.003). Incorporating netrin-1 to conventional risk factors improved the discriminatory power for ischemic stroke (net reclassification index = 98.0%, P < 0.001; integrated discrimination improvement = 0.28%, P = 0.027).

CONCLUSIONS:

Serum netrin-1 was decreased in patients with ischemic stroke compared with healthy controls, suggesting that there was a potential role of netrin-1 in the pathogenesis of ischemic stroke.

• Authors: Daoxia Guo, Ying Qiao, Zaihan Li, Zhengbao Zhu, Hao Peng, Qiu Zhang, Chongke Zhong, Aili Wang, Tan Xu, Jing Chen, Yonghong Zhang, Jiang He
• Journal: Nutrition, metabolism, and cardiovascular diseases : NMCD (Nutr Metab Cardiovasc Dis) Vol. 30 Issue 12 Pg. 2328-2334 (11 27 2020) ISSN: 1590-3729 [Electronic] Netherlands
• PMID: 32917498 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020. Published by Elsevier B.V.

Chemical References

• Biomarkers
• NTN1 protein, human
• Netrin-1

Topics

• Adult
• Aged
• Biomarkers (blood)
• Brain Ischemia (blood, diagnosis)
• Case-Control Studies
• Down-Regulation
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Male
• Middle Aged
• Netrin-1 (blood)
• Predictive Value of Tests
• Prognosis
• Randomized Controlled Trials as Topic
• Stroke (blood, diagnosis)