Hericium erinaceus potentially rescues behavioural motor deficits through ERK-CREB-PSD95 neuroprotective mechanisms in rat model of 3-acetylpyridine-induced cerebellar ataxia.

Abstract

Cerebellar ataxia is a neurodegenerative disorder with no definitive treatment. Although several studies have demonstrated the neuroprotective effects of Hericium erinaceus (H.E.), its mechanisms in cerebellar ataxia remain largely unknown. Here, we investigated the neuroprotective effects of H.E. treatment in an animal model of 3-acetylpyridine (3-AP)-induced cerebellar ataxia. Animals administered 3-AP injection exhibited remarkable impairments in motor coordination and balance. There were no significant effects of 25 mg/kg H.E. on the 3-AP treatment group compared to the 3-AP saline group. Interestingly, there was also no significant difference in the 3-AP treatment group compared to the non-3-AP control, indicating a potential rescue of motor deficits. Our results revealed that 25 mg/kg H.E. normalised the neuroplasticity-related gene expression to the level of non-3-AP control. These findings were further supported by increased protein expressions of pERK1/2-pCREB-PSD95 as well as neuroprotective effects on cerebellar Purkinje cells in the 3-AP treatment group compared to the 3-AP saline group. In conclusion, our findings suggest that H.E. potentially rescued behavioural motor deficits through the neuroprotective mechanisms of ERK-CREB-PSD95 in an animal model of 3-AP-induced cerebellar ataxia.

• Authors: Pit Shan Chong, Sharafuddin Khairuddin, Anna Chung Kwan Tse, Lih Fhung Hiew, Chun Lok Lau, George Lim Tipoe, Man-Lung Fung, Kah Hui Wong, Lee Wei Lim
• Journal: Scientific reports (Sci Rep) Vol. 10 Issue 1 Pg. 14945 (09 10 2020) ISSN: 2045-2322 [Electronic] England
• PMID: 32913245 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclic AMP Response Element-Binding Protein
• Disks Large Homolog 4 Protein
• Neuroprotective Agents
• Neurotoxins
• Pyridines
• 3-acetylpyridine
• Extracellular Signal-Regulated MAP Kinases

Topics

• Animals
• Behavior, Animal (drug effects)
• Cerebellar Ataxia (chemically induced, drug therapy, psychology)
• Cyclic AMP Response Element-Binding Protein (genetics, metabolism)
• Disease Models, Animal
• Disks Large Homolog 4 Protein (genetics, metabolism)
• Extracellular Signal-Regulated MAP Kinases (genetics, metabolism)
• Hericium (chemistry, growth & development)
• Male
• Motor Disorders (genetics, metabolism, pathology, prevention & control)
• Neuroprotective Agents (pharmacology)
• Neurotoxins (toxicity)
• Purkinje Cells (drug effects, pathology)
• Pyridines (toxicity)
• Rats
• Rats, Sprague-Dawley