Galangin ameliorated pulmonary fibrosis in vivo and in vitro by regulating epithelial-mesenchymal transition.

Abstract

Pulmonary fibrosis (PF) is a disease that is characterized by abnormal epithelial-mesenchymal transition (EMT) and persistent inflammatory injury, with high mortality and poor prognosis, but the current therapies are accompanied by certain adverse side effects. In this study, we investigated the role of galangin (GA), an anti-inflammatory and anti-tumoral phytochemical extracted from galangal, in preventing and curing bleomycin (BLM)-induced pulmonary fibrosis and the underlying mechanism. Histopathological staining confirmed that GA dramatically moderated bleomycin-induced pulmonary fibrosis in mice. Compared with the vehicle treatment, GA treatment inhibited the expression of vimentin and increased the expression of E-cadherin. The expression of α-Smooth muscle actin (α-SMA), which is a myofibroblast marker, was also suppressed. In addition, GA diminished the increase in the numbers of CD4+CD69+ and CD8+CD69+ T cells and dendritic cells induced by bleomycin, and reduced the residence of inflammatory cells in the lung tissues. Notably, GA inhibited the TGF-β1-induced EMT and fibroblast differentiation in vitro, which further confirmed the potential protective effect of GA on pulmonary fibrosis. Taken together, our results suggest that GA exerts a beneficial effect on bleomycin-induced pulmonary fibrosis by attenuating EMT and inflammatory damage and may have prevent potential of pulmonary fibrosis.

• Authors: Liqun Wang, Hongyao Liu, Qiurong He, Cailing Gan, Yali Li, Qianyu Zhang, Yuqin Yao, Fang He, Tinghong Ye, Wenya Yin
• Journal: Bioorganic & medicinal chemistry (Bioorg Med Chem) Vol. 28 Issue 19 Pg. 115663 (10 01 2020) ISSN: 1464-3391 [Electronic] England
• PMID: 32912432 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Antineoplastic Agents, Phytogenic
• Flavonoids
• Phytochemicals
• Bleomycin
• galangin

Topics

• A549 Cells
• Animals
• Anti-Inflammatory Agents (chemistry, isolation & purification, pharmacology)
• Antineoplastic Agents, Phytogenic (chemistry, isolation & purification, pharmacology)
• Bleomycin
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Epithelial-Mesenchymal Transition (drug effects)
• Flavonoids (chemistry, isolation & purification, pharmacology)
• Humans
• Male
• Mice
• Mice, Inbred C57BL
• Molecular Structure
• Phytochemicals (chemistry, isolation & purification, pharmacology)
• Pulmonary Fibrosis (chemically induced, drug therapy)
• Structure-Activity Relationship
• Tumor Cells, Cultured