Prevention of bone infection after open fracture using a chitosan with ciprofloxacin implant in animal model.

Abstract

PURPOSE:

To evaluate different concentrations of ciprofloxacin to prevent infection after open fracture contaminated with S. aureus in rats using absorbable local delivery system.

METHODS:

Fifty-two Wistar rats were assigned to six groups. After 4 weeks, all animals underwent 99mTc-ceftizoxima scintigraphy evaluation, callus formation measurement and histological analysis. ANOVA, t-Student and Kruskal Wallis were used for quantitative variables statistical analysis, whereas qui square and exact Fisher were used for qualitative variables.

RESULTS:

Treatment using 25% and 50% of ciprofloxacin incorporated at the fracture fixation device were effective in preventing bone infection compared to control group (p<0.05). Chitosan were not effective in preventing bone infection when used alone compared to control group (p>0.05). Histological findings demonstrated bone-healing delay with 50% of ciprofloxacin. No difference in callus formation were observed (p>0.05).

CONCLUSION:

Local delivery treatment for contaminated open fracture using chitosan with ciprofloxacin is effective above 25%.

• Authors: Silvia Iovine Kobata, Luiz Eduardo Moreira Teixeira, Simone Odilia Antunes Fernandes, André Augusto Gomes Faraco, Paula Vieira Teixeira Vidigal, Ivana Duval de Araújo
• Journal: Acta cirurgica brasileira (Acta Cir Bras) Vol. 35 Issue 8 Pg. e202000803 (Sep 04 2020) ISSN: 1678-2674 [Electronic] Brazil
• PMID: 32901680 (Publication Type: Journal Article)

Chemical References

• Ciprofloxacin
• Chitosan

Topics

• Animals
• Bony Callus
• Chitosan (therapeutic use)
• Ciprofloxacin (therapeutic use)
• Femoral Fractures (complications, surgery)
• Fracture Healing
• Fractures, Open
• Humans
• Infection Control
• Infections
• Rats
• Rats, Wistar
• Staphylococcus aureus