Desflurane protects against liver ischemia/reperfusion injury via regulating miR-135b-5p.

Abstract

BACKGROUND:

A number of anesthetics have protective effect against ischemia-reperfusion (I/R) injury, including desflurane. But the function and molecular mechanism of desflurane in liver I/R injury have not been fully understood. The aim of this study was to investigate the effect of desflurane on liver I/R injury and further investigated the molecular mechanisms involving in miR-135b-5p.

METHODS:

The models of liver I/R injury in rats were established, and received desflurane treatment throughout the injury. Serum alanine transaminase (ALT) and aspartate transaminase (AST) were measured and compared between groups. H/R-induced cell model in L02 was established, and were treated with desflurane before hypoxia. Quantitative real-time polymerase chain reaction was performed to determine the expression of miR-135b-5p in different groups. The cell apoptosis was detected using flow cytometry assay. Western blot was used for the measurement of protein levels.

RESULTS:

I/R significantly increased serum levels of ALT and AST in rats, which were reversed by desflurane treatment. Desflurane also significantly attenuated the increase of cell apoptosis induced by I/R in both vivo and vitro. MiR-135b-5p significantly reversed the protective effect of desflurane against liver I/R injury. Additionally, Janus protein tyrosine kinase (JAK)2 was shown to be a target gene of miR-135b-5p, and miR-135b-5p overexpression significantly decreased the protein levels of p-JAK2, JAK2, p-STAT3.

CONCLUSION:

Desflurane attenuated liver I/R injury through regulating miR-135b-5p, and JAK2 was the target gene of mIR-135b-5p. These findings provide references for further development of therapeutic strategies in liver injury.

• Authors: Mengxia Zhong, Lei Che, Meiqing Du, Ke Liu, Dalong Wang
• Journal: Journal of the Chinese Medical Association : JCMA (J Chin Med Assoc) Vol. 84 Issue 1 Pg. 38-45 (Jan 01 2021) ISSN: 1728-7731 [Electronic] Netherlands
• PMID: 32898087 (Publication Type: Journal Article)
• Copyright: Copyright © 2020, the Chinese Medical Association.

Chemical References

• MIRN135 microRNA, human
• MIRN135 microRNA, rat
• MicroRNAs
• STAT Transcription Factors
• Desflurane
• Janus Kinase 2

Topics

• Animals
• Cells, Cultured
• Desflurane (therapeutic use)
• Humans
• Janus Kinase 2 (genetics, physiology)
• Liver (blood supply)
• Male
• MicroRNAs (physiology)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (prevention & control)
• STAT Transcription Factors (physiology)