Abstract | OBJECTIVE: To investigate the role of cholinergic anti-inflammatory pathway (CAP) in neuro-regulation of inflammatory and immune response in the early stage of sepsis. METHODS: RESULTS: Compared with the control rats, the septic rats had significantly increased MSS scores and lowered HRV indexes (SDNN, RMSSD, HF, SD1, and SD2; P < 0.05); treatment with GTS-21 significantly decreased while MLA increased MSS scores (P < 0.05), but neither of them obviously affected HRV of the rats. Serum levels TNF-α, IL-1α, IL-10, IL-6, HMGB1, and sCD14 and the percentages of CD4+CD25+ Treg and TH17-positive lymphocytes were significantly higher and Treg/TH17 ratio was significantly lower in the septic rats compared with those in the control group (P < 0.05); treatment with GTS-21 significantly decreased the levels of serum levels of TNF-α, IL-1α, IL-6, HMGB1, and sCD14 and TH17 lymphocyte percentage (P < 0.05), whereas MLA treatment significantly increased serum levels of TNF-α, IL-1α, IL-10, IL-6, HMGB1, and sCD14 and the percentages of CD4+ CD25+ Treg and TH17-positive lymphocytes and decreased Treg/TH17 ratio in the septic rats (P < 0.05). CONCLUSIONS: CAP plays negative regulatory role in early inflammatory and immune response to sepsis, and some of the HRV indicators can well reflect the regulatory effect of CAP on inflammation and immunity in the septic rats.
|
Authors | Hongbing Li, Yuan Li, Wushi Wang, Zongshuang Peng, Fang Wu |
Journal | Nan fang yi ke da xue xue bao = Journal of Southern Medical University
(Nan Fang Yi Ke Da Xue Xue Bao)
Vol. 40
Issue 5
Pg. 647-653
(May 30 2020)
ISSN: 1673-4254 [Print] China |
PMID | 32897210
(Publication Type: Journal Article)
|
Topics |
- Animals
- Disease Models, Animal
- Mice
- Neuroimmunomodulation
- Rats
- Rats, Sprague-Dawley
- Sepsis
- T-Lymphocytes, Regulatory
|