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Diagnostic test assessment. Validation study of an alternative system to detect microsatellite instability in colorectal carcinoma.

Abstract
The American Society for Clinical Pathology (ASCP), College of American Pathologists (CAP), Association for Molecular Pathology (AMP), and the American Society of Clinical Oncology (ASCO) have been recently strongly recommended the evaluation of mismatch repair status (MMS) as molecular biomarkers in colorectal cancer for a better prognostic stratification of patients. This recommendation is emphasized by the recent evidence of Microsatellite Instability (MSI) as a predictive marker for chemotherapy and immunotherapy.
In this scenario, the validation of molecular biomarker testing methods seems to be essential to design the most appropriate tailored therapy and the most suitable care strategy, respectively.
In this study, we validated an alternative method based on capillary electrophoresis system label-free PCR (Qiaxcel system) to evaluate the MSI Bethesda Panel. We also parallel the results with a standard approach.
Our data showed total concordance with the standard approach, with a highly time-efficient and easy procedure combined with high sensitivity for MSI detection.
Alternative capillary electrophoresis based on label-free PCR such as the Qiaxel system is a very sensitive and specific method to detect MSI for the management of patients with colorectal cancer. This procedure is adequate and suitable in diagnostic routine for the evaluation of microsatellite repeats compared to standard procedures.
AuthorsSimona Vatrano, Angela Pettinato, Valentina Randazzo, Marina Zagami, Cecilia Agueli, Sonia Cannella, Giuseppe Luigi Banna, Filippo Fraggetta, Alessandra Santoro
JournalPathologica (Pathologica) Vol. 112 Issue 4 Pg. 178-183 (Dec 2020) ISSN: 1591-951X [Electronic] Italy
PMID32893846 (Publication Type: Journal Article)
CopyrightCopyright © 2020 Società Italiana di Anatomia Patologica e Citopatologia Diagnostica, Divisione Italiana della International Academy of Pathology.
Chemical References
  • Biomarkers, Tumor
  • DNA, Neoplasm
Topics
  • Biomarkers, Tumor (genetics)
  • Colorectal Neoplasms (diagnosis, genetics, therapy)
  • DNA Mismatch Repair (genetics)
  • DNA, Neoplasm (analysis)
  • Diagnostic Tests, Routine (methods)
  • Drug Therapy
  • Humans
  • Immunotherapy
  • Microsatellite Instability
  • Pathology, Molecular

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