Abstract |
Matching of symmetry at interfaces is a fundamental obstacle in molecular assembly. Virus-like particles (VLPs) are important vaccine platforms against pathogenic threats, including Covid-19. However, symmetry mismatch can prohibit vaccine nanoassembly. We established an approach for coupling VLPs to diverse antigen symmetries. SpyCatcher003 enabled efficient VLP conjugation and extreme thermal resilience. Many people had pre-existing antibodies to SpyTag:SpyCatcher but less to the 003 variants. We coupled the computer-designed VLP not only to monomers (SARS-CoV-2) but also to cyclic dimers ( Newcastle disease, Lyme disease), trimers ( influenza hemagglutinins), and tetramers ( influenza neuraminidases). Even an antigen with dihedral symmetry could be displayed. For the global challenge of influenza, SpyTag-mediated display of trimer and tetramer antigens strongly induced neutralizing antibodies. SpyCatcher003 conjugation enables nanodisplay of diverse symmetries towards generation of potent vaccines.
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Authors | Rolle Rahikainen, Pramila Rijal, Tiong Kit Tan, Hung-Jen Wu, Anne-Marie C Andersson, Jordan R Barrett, Thomas A Bowden, Simon J Draper, Alain R Townsend, Mark Howarth |
Journal | Angewandte Chemie (International ed. in English)
(Angew Chem Int Ed Engl)
Vol. 60
Issue 1
Pg. 321-330
(01 04 2021)
ISSN: 1521-3773 [Electronic] Germany |
PMID | 32886840
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2020 The Authors. Published by Wiley-VCH GmbH. |
Chemical References |
- Antibodies, Neutralizing
- Antibodies, Viral
- Antigens, Viral
- COVID-19 Vaccines
- Vaccines, Virus-Like Particle
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Topics |
- Antibodies, Neutralizing
(analysis)
- Antibodies, Viral
- Antigens, Viral
(chemistry, immunology)
- COVID-19 Vaccines
(chemistry)
- Freezing
- Humans
- Models, Molecular
- Nanostructures
(chemistry)
- Vaccines, Virus-Like Particle
(chemistry)
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