Abstract |
The aim of the present study was to determine the effect of microRNA (miR)-132 on cardiac fibrosis in myocardial infarction (MI)-induced heart failure and angiotensin (Ang) II-treated cardiac fibroblasts (CFs). Experiments were carried out in Sprague-Dawley rat treatment with ligation of left coronary artery to induce heart failure, and in CFs administration of Ang II to induce fibrosis. The level of miR-132 was increased in the heart of rats with MI-induced heart failure and the Ang II-treated CFs. In MI rats, left ventricle (LV) ejection fraction, fractional shortening, the maximum of the first differentiation of LV pressure (LV +dp/dtmax) and decline (LV -dp/dtmax) and LV systolic pressure (LVSP) were reduced, and LV end-systolic diameter (LVESD), LV end-diastolic diameter (LVEDD), LV volumes in systole (LVVS) and LV volumes in diastole (LVVD) were increased, which were reversed by miR-132 agomiR but deteriorated by miR-132 antagomiR. The expression levels of collagen I, collagen III, transforming growth factor-β (TGF-β), and α-smooth muscle actin (α-SMA) were increased in the heart of rat with MI-induced heart failure and CFs administration of Ang II. These increases were inhibited by miR-132 agomiR but enhanced by miR-132 antagomiR treatment. MiR-132 inhibited PTEN expression, and attenuated PI3K/Akt signal pathway in CFs. These results indicated that the up-regulation of miR-132 improved the cardiac dysfunction, attenuated cardiac fibrosis in heart failure via inhibiting PTEN expression, and attenuating PI3K/Akt signal pathway. Up-regulation of miR-132 may be a strategy for the treatment of heart failure and cardiac fibrosis.
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Authors | Guoyu Wang, Ruzhu Wang, Zhongbao Ruan, Ling Liu, Yong Li, Li Zhu |
Journal | Bioscience reports
(Biosci Rep)
Vol. 40
Issue 9
(09 30 2020)
ISSN: 1573-4935 [Electronic] England |
PMID | 32885809
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2020 The Author(s). |
Chemical References |
- Cardiotonic Agents
- MIRN132 microRNA, rat
- MicroRNAs
- Angiotensin II
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Topics |
- Angiotensin II
(administration & dosage)
- Animals
- Cardiotonic Agents
(pharmacology, therapeutic use)
- Cells, Cultured
- Disease Models, Animal
- Echocardiography
- Fibroblasts
(drug effects, pathology)
- Fibrosis
- Heart
(drug effects)
- Heart Failure
(diagnosis, drug therapy, etiology, pathology)
- Humans
- Male
- MicroRNAs
(agonists, antagonists & inhibitors, metabolism)
- Myocardial Infarction
(chemically induced, complications, diagnosis, pathology)
- Myocardium
(cytology, pathology)
- Primary Cell Culture
- Rats
- Signal Transduction
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